Goat anti-Mouse IgG1 (Heavy Chain) Antibody (Texas Red (TR)) - Preadsorbed
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- Target See all IgG1 products
- IgG1
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Binding Specificity
- Heavy Chain
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Reactivity
- Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Texas Red (TR)
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Specificity
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Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG.
Specificity was confirmed by ELISA at less than 0.5 % of target signal. - Cross-Reactivity
- Mouse (Murine)
- Characteristics
- Mouse IgG1 Antibody Texas Red™ Conjugated Pre-Adsorbed antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Mouse IgG1 heavy chain
Immunogen Type: Native Protein
- Isotype
- IgG1
- Labeling Ratio
- 5.8
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- Application Notes
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Mouse IgG1 secondary antibodies are suitable for ELISA, FLISA, Flow Cytometry, Immunohistochemistry western blotting as well as other anti-IgG1 antibody based assays.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
Western Blot Dilution: 1:4,000 - 1:20,000
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Comment
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Texas Red™ is a registered trademark of Molecular Probes Inc.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Product is photosensitive and should be protected from light.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- This product is stable for several weeks at 4 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -24 °C or below.
- Expiry Date
- 12 months
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- Target
- IgG1
- Abstract
- IgG1 Products
- Target Type
- Antibody
- Background
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Synonyms: Goat anti-mouse IgG1 antibody Texas Red™ conjugation, goat anti-mouse IgG1 (gamma 1) Texas Red™ conjugated antibody
Background: Anti-Mouse IgG1 Texas Red Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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