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ICAM-1 antibody (Intercellular Adhesion Molecule 1)

Details for Product anti-ICAM1 Antibody No. ABIN111934, Supplier: Log in to see
Antigen
  • BB2
  • CD54
  • P3.58
  • Icam-1
  • Ly-47
  • MALA-2
  • ICAM
  • ICAM-1
  • ICAM1
Reactivity
Human
580
145
108
11
10
7
1
1
1
Host
Mouse
418
253
77
10
8
5
4
2
Clonality (Clone)
Monoclonal ()
Conjugate
This ICAM-1 antibody is un-conjugated
77
77
68
20
10
10
8
8
6
4
4
4
4
4
4
3
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
381
297
187
159
148
95
94
50
49
34
31
12
12
10
9
9
6
6
5
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Immunogen Cultured Human endothelial cells.
Clone 1304
Isotype IgG2a
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Protein A Chromatography
Alternative Name CD54 / ICAM1 (ICAM1 Antibody Abstract)
Background ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.Synonyms: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor
Gene ID 9606
UniProt P05362
Pathways
Application Notes Suitable for Immunohistochemistry (IHC), FACS and Western Blots. Recommended Dilutions for IHC: Frozen sections: 0.25 μg/mL (1/800). Paraffin sections: 2 μg/mL (1/100), Proteinase K pretreatment for antigen retrieval isrecommended. Suggested positive control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water4. Block endogenous peroxidase5. Wash in PBS
Restrictions For Research Use only
Reconstitution Restore with 0.5 mL distilled water.
Concentration 0.2 mg/mL
Buffer PBS pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Rothlein, Dustin, Marlin et al.: "A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 4, pp. 1270-4, 1986 (PubMed).

Albelda, Buck: "Integrins and other cell adhesion molecules." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 4, Issue 11, pp. 2868-80, 1990 (PubMed).

Freudenthal, Steinman: "The distinct surface of human blood dendritic cells, as observed after an improved isolation method." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 19, pp. 7698-702, 1990 (PubMed).

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, 1990 (PubMed).