Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
Specificity
Suitable for analysis of LDL Receptor function in patients with familial hypercholesterolemia. The antibody recognizes an epitope in the region of repeat #1 of the ligand binding region. Addition of 15 nM antibody results in inhibition of half-maximal LDL-binding (cf. Beisiegel et al. 1981). In Human normal fibroblasts the antibody detects the 160 kDa polypeptide (pI 4.3) and also in bovine adrenal gland (160 kDa, pI 4.6) of LDL receptors (Beisiegel et al. 1982).
LDLR
Reactivity: Rat
WB, IHC, ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
FACS. Immunoblotting (Western blotting). Immunofluorescence (1/10-1/50). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 1.0 mL distilled water.
Buffer
Final solution contains PBS buffer, pH 7.4 with 0.5 % BSA as stabilizer
Handling Advice
This product is photosensitive and should be protected from light
Storage
4 °C
Storage Comment
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
FH antibody, FHC antibody, LDLCQ2 antibody, Hlb301 antibody, LDLRA antibody, LDLA antibody, LDL receptor-2 antibody, fhc antibody, ldlcq2 antibody, ldlr antibody, ldlr2-a antibody, LDL receptor 1 antibody, ldlr-a antibody, ldlr-b antibody, low density lipoprotein receptor antibody, low density lipoprotein receptor a antibody, low density lipoprotein receptor S homeolog antibody, low density lipoprotein receptor L homeolog antibody, LDLR antibody, Ldlr antibody, ldlra antibody, ldlr.S antibody, ldlr.L antibody
Background
The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia.Synonyms: LDL receptor, LDLR, Low-density lipoprotein receptor