PG 5.1 represents an excellent marker for all forms of intercellular adhering junctions, such as: Desmosomes of epithelial and myocardial cells (incl. cultured cells), Zonulae and fasciae adherentes of epithelia, endothelia of blood vessels and myocardial cells, adherens-type junctions (e.g. lens tissue, pigmented retinal cells, Sertoli cells of testis). The PG 5.1 epitope maps within the C-terminus at the extreme end of repeat 13 (aa 632-687) of plakoglobin. Polypeptide Reacting: Mr 83 000 Plakoglobin, "band 5" polypeptide of intercellular adhering junctions (identical to γ-catenin). Reactivity on Cultured Cell Lines: Cell cultures forming monolayers (from tested species cited below).
Cross-Reactivity (Details)
Species reactivity (tested):Human, Bovine, Rat, Mouse, Chicken and Zebrafish.
Purification
Affinity Chromatography on Protein A
Immunogen
Plakoglobin, "band 5" protein from Bovine snout epidermis
Immunoblotting. Immunoprecipitation. Immunofluorescence Microscopy: 1/5 (1 h at RT). Immunohistochemistry on Frozen Sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in 1 mL distilled water
Buffer
Final solution contains PBS buffer, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Gamma Catenin is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of gamma Catenin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. It is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat.Synonyms: CTNNG, Catenin gamma, DP3, Desmoplakin III, Desmoplakin-3, JUP