Leptin antibody (LEP)

Details for Product anti-LEP Antibody No. ABIN115496, Supplier: Log in to see
Antigen
  • ob
  • obese
  • LEPD
  • OB
  • OBS
  • leptin
  • Lep
  • LEP
  • lep
Alternatives
anti-Mouse (Murine) Leptin antibody for Blocking Antibody
Reactivity
Mouse (Murine)
417
171
152
24
7
6
5
4
4
4
3
3
2
2
1
1
1
1
1
1
Host
Rabbit
367
207
19
8
4
1
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
64
26
17
14
10
7
7
7
6
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
406
400
107
57
56
39
31
30
29
25
17
10
10
6
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Immunogen The immunization antigen (16 kDa) is a protein containing 146 amino acid residues of the mouse Leptin and 23 additional amino acid residues - (underlined).
Specificity The antibody was raised in rabbits by immunization with the recombinant Leptin.
Purification Immunoaffinity chromatography
Alternative Name Leptin (LEP Antibody Abstract)
Background Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa proteohormone consisting of 146 amino acid residues. Leptin is produced by differentiated adiocytes, although production have been demonstrated in other tissues, such as fundus of the stomach, the sceletal muscle, the liver, and the placenta. Leptin is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, in particular the hypothalamus, suppressing food intake and stimulating energy expenditure. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action, it decreases the appetite and increases energy consumption, causing more fat to be burned.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor
Gene ID 16846
NCBI Accession NP_032519
UniProt P41160
Research Area Cardiovascular, Atherosclerosis, Hormones
Pathways JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
Application Notes ELISA. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 0.1 mL of deionized water
Concentration 1.0 mg/mL
Buffer 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
Expiry Date 6 months
Background publications Ricci, Lee, Russell, Wang, Sullivan, Schneider, Brolin, Fried: "Isoproterenol decreases leptin release from rat and human adipose tissue through posttranscriptional mechanisms." in: American journal of physiology. Endocrinology and metabolism, Vol. 288, Issue 4, pp. E798-804, 2005 (PubMed).

Considine, Sinha, Heiman, Kriauciunas, Stephens, Nyce, Ohannesian, Marco, McKee, Bauer: "Serum immunoreactive-leptin concentrations in normal-weight and obese humans." in: The New England journal of medicine, Vol. 334, Issue 5, pp. 292-5, 1996 (PubMed).

Maffei, Halaas, Ravussin, Pratley, Lee, Zhang, Fei, Kim, Lallone, Ranganathan: "Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects." in: Nature medicine, Vol. 1, Issue 11, pp. 1155-61, 1995 (PubMed).

Lönnqvist, Arner, Nordfors, Schalling: "Overexpression of the obese (ob) gene in adipose tissue of human obese subjects." in: Nature medicine, Vol. 1, Issue 9, pp. 950-3, 1995 (PubMed).

Halaas, Gajiwala, Maffei, Cohen, Chait, Rabinowitz, Lallone, Burley, Friedman: "Weight-reducing effects of the plasma protein encoded by the obese gene." in: Science (New York, N.Y.), Vol. 269, Issue 5223, pp. 543-6, 1995 (PubMed).

Zhang, Proenca, Maffei, Barone, Leopold, Friedman: "Positional cloning of the mouse obese gene and its human homologue." in: Nature, Vol. 372, Issue 6505, pp. 425-32, 1994 (PubMed).