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Leptin antibody (LEP)

Details for Product anti-LEP Antibody No. ABIN115496, Supplier: Log in to see
Antigen
  • LEPD
  • OB
  • ob
  • obese
  • OBS
Alternatives
anti-Mouse (Murine) Leptin antibody for ELISA
Reactivity
Mouse (Murine)
412
151
132
18
3
2
2
1
Host
Rabbit
349
213
20
8
4
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
65
23
17
11
7
7
7
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
414
381
71
59
41
33
25
25
19
17
16
14
10
7
5
3
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Immunogen The immunization antigen (16 kDa) is a protein containing 146 amino acid residues of the mouse Leptin and 23 additional amino acid residues - (underlined).
Specificity The antibody was raised in rabbits by immunization with the recombinant Leptin.
Purification Immunoaffinity chromatography
Alternative Name Leptin (LEP Antibody Abstract)
Background Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa proteohormone consisting of 146 amino acid residues. Leptin is produced by differentiated adiocytes, although production have been demonstrated in other tissues, such as fundus of the stomach, the sceletal muscle, the liver, and the placenta. Leptin is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, in particular the hypothalamus, suppressing food intake and stimulating energy expenditure. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action, it decreases the appetite and increases energy consumption, causing more fat to be burned.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor
Gene ID 16846
NCBI Accession NP_032519
UniProt P41160
Research Area Cardiovascular, Atherosclerosis, Hormones
Pathways JAK-STAT Signaling, AMPK Signaling
Application Notes ELISA. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 0.1 mL of deionized water
Concentration 1.0 mg/mL
Buffer 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
Expiry Date 6 months
Background publications Ricci, Lee, Russell, Wang, Sullivan, Schneider, Brolin, Fried: "Isoproterenol decreases leptin release from rat and human adipose tissue through posttranscriptional mechanisms." in: American journal of physiology. Endocrinology and metabolism, Vol. 288, Issue 4, pp. E798-804, 2005 (PubMed).

Considine, Sinha, Heiman, Kriauciunas, Stephens, Nyce, Ohannesian, Marco, McKee, Bauer: "Serum immunoreactive-leptin concentrations in normal-weight and obese humans." in: The New England journal of medicine, Vol. 334, Issue 5, pp. 292-5, 1996 (PubMed).

Maffei, Halaas, Ravussin, Pratley, Lee, Zhang, Fei, Kim, Lallone, Ranganathan: "Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects." in: Nature medicine, Vol. 1, Issue 11, pp. 1155-61, 1995 (PubMed).

Lönnqvist, Arner, Nordfors, Schalling: "Overexpression of the obese (ob) gene in adipose tissue of human obese subjects." in: Nature medicine, Vol. 1, Issue 9, pp. 950-3, 1995 (PubMed).

Halaas, Gajiwala, Maffei, Cohen, Chait, Rabinowitz, Lallone, Burley, Friedman: "Weight-reducing effects of the plasma protein encoded by the obese gene." in: Science (New York, N.Y.), Vol. 269, Issue 5223, pp. 543-6, 1995 (PubMed).

Zhang, Proenca, Maffei, Barone, Leopold, Friedman: "Positional cloning of the mouse obese gene and its human homologue." in: Nature, Vol. 372, Issue 6505, pp. 425-32, 1994 (PubMed).