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Endoglin (ENG) (AA 22-586) antibody

Details for Product No. ABIN115697, Supplier: Log in to see
Antigen
  • AI528660
  • AI662476
  • CD105
  • DKFZp469D0419
  • END
  • ENG
  • HHT1
  • MGC137842
  • ORW1
  • S-endoglin
Epitope
AA 22-586
23
23
16
14
13
12
12
11
11
10
9
8
8
7
7
6
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
401
166
52
16
11
7
5
5
5
2
1
1
1
Host
Rabbit
237
199
91
3
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
57
51
41
21
8
6
6
5
5
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)
309
298
126
124
101
62
50
44
30
12
11
11
9
7
6
4
3
3
3
2
2
2
2
2
1
1
1
1
Supplier
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Immunogen Recombinant Human soluble CD105/Endoglin (aa. 22 (Glu) to 586 (Leu)) derived from Insect Cells
Purification Protein A Chromatography
Alternative Name CD105 / Endoglin (ENG Antibody Abstract)
Background Endoglin, also known as CD105, is a Type I integral membrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Two splice variants of human endoglin, the S-endoglin and L-endoglin that differ in the length of their cytoplasmic tails have been identified. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, bone marrow pro-erythroblasts, and leukemic cells of lymphoid and myeloid lineages. Human and mouse endoglin share approximately 70 % and 97 % amino acid sequence identity in their extracellular and intracellular domains, respectively. It has clearly been shown that CD105/Endoglin is required for angiogenesis and it plays a key role in heart development. Mutations in human endoglin or ALK-1 (another type I serine/threonine receptor) lead to the vascular disorder hereditary hemorrhagic telangiectasia (HHT). Mice heterozygous for endoglin have been developed as disease models for HHT. Endoglin has been shown to be a powerful marker of neovascularization. It is also useful as a functional marker that defines long-term repopulating hematopoietic stem cells.Synonyms: END, ENG, HHT1, ORW, ORW1
Gene ID 9606
UniProt P17813
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Elisa (1-15 μg/mL). Western blot (1-2 μg/mL with the appropriate secondary reagents). FACS analysis and cell sorting (2-5 μg/mL together with the appropriate secondaryreagents).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL
Buffer PBS, pH 7.4 without preservatives
Preservative Without preservative
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Background publications Parker, Goldring, Philip: "Endoglin is expressed on human chondrocytes and forms a heteromeric complex with betaglycan in a ligand and type II TGFbeta receptor independent manner." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 18, Issue 2, pp. 289-302, 2003

Fonsatti, Del Vecchio, Altomonte, Sigalotti, Nicotra, Coral, Natali, Maio: "Endoglin: An accessory component of the TGF-beta-binding receptor-complex with diagnostic, prognostic, and bioimmunotherapeutic potential in human malignancies." in: Journal of cellular physiology, Vol. 188, Issue 1, pp. 1-7, 2001

Arthur, Ure, Smith, Renforth, Wilson, Torsney, Charlton, Parums, Jowett, Marchuk, Burn, Diamond: "Endoglin, an ancillary TGFbeta receptor, is required for extraembryonic angiogenesis and plays a key role in heart development." in: Developmental biology, Vol. 217, Issue 1, pp. 42-53, 2000

Barbara, Wrana, Letarte: "Endoglin is an accessory protein that interacts with the signaling receptor complex of multiple members of the transforming growth factor-beta superfamily." in: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 584-94, 1999

McAllister, Grogg, Johnson, Gallione, Baldwin, Jackson, Helmbold, Markel, McKinnon, Murrell: "Endoglin, a TGF-beta binding protein of endothelial cells, is the gene for hereditary haemorrhagic telangiectasia type 1." in: Nature genetics, Vol. 8, Issue 4, pp. 345-51, 1995

Cheifetz, Bellón, Calés, Vera, Bernabeu, Massagué, Letarte: "Endoglin is a component of the transforming growth factor-beta receptor system in human endothelial cells." in: The Journal of biological chemistry, Vol. 267, Issue 27, pp. 19027-30, 1992