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Endoglin (ENG) (AA 26-581) antibody

Details for Product No. ABIN115701, Supplier: Log in to see
Antigen
  • AI528660
  • AI662476
  • CD105
  • DKFZp469D0419
  • END
  • ENG
  • HHT1
  • MGC137842
  • ORW1
  • S-endoglin
Epitope
AA 26-581
23
23
16
14
13
12
12
11
11
10
10
8
8
8
7
7
7
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
463
173
67
30
11
7
5
5
5
2
1
1
1
Host
Rabbit
291
206
99
3
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
60
52
43
21
13
10
7
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
367
347
159
128
118
89
61
58
46
12
11
11
9
7
6
4
3
3
3
3
2
2
2
2
1
1
1
1
1
Supplier
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Immunogen Recombinant mouse soluble CD105/Endoglin. It consists of amino acid 26 (Glu) to 581 (Gly) and is fused to a C-terminal His-tag (6xHis).
Purification Antigen affinity chromatography
Alternative Name CD105 / Endoglin (ENG Antibody Abstract)
Background Endoglin, also known as CD105, is a Type I integral membrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Two splice variants of human endoglin, the S-endoglin and L-endoglin that differ in the length of their cytoplasmic tails have been identified. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, bone marrow pro-erythroblasts, and leukemic cells of lymphoid and myeloid lineages. Human and mouse endoglin share approximately 70 % and 97 % amino acid sequence identity in their extracellular and intracellular domains, respectively. It has clearly been shown that CD105/Endoglin is required for angiogenesis and it plays a key role in heart development. Mutations in human endoglin or ALK-1 (another type I serine/threonine receptor) lead to the vascular disorder hereditary hemorrhagic telangiectasia (HHT). Mice heterozygous for endoglin have been developed as disease models for HHT. Endoglin has been shown to be a powerful marker of neovascularization. It is also useful as a functional marker that defines long-term repopulating hematopoietic stem cells.Synonyms: END, ENG, HHT1, ORW, ORW1
Gene ID 10090
UniProt Q63961
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes ELISA (1 - 15 μg/mL). Western blot (1 - 2 μg/mL). Flow cytometry (3 - 20 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Reconstitute in sterile water/PBS to a concentration of >0.5 mg/mL
Buffer Immunglobulin fraction in Phosphate buffered saline pH 7.4 without preservative and stabilizer
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles. Following reconstitution the antibody is stable for six weeks at 2-8°C.
Storage 4 °C/-20 °C
Storage Comment The lyophilized IgG is stable at 2-8 °C for one month and for one year from despatch when kept at -20 °C.
Expiry Date 12 months
Background publications Parker, Goldring, Philip: "Endoglin is expressed on human chondrocytes and forms a heteromeric complex with betaglycan in a ligand and type II TGFbeta receptor independent manner." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 18, Issue 2, pp. 289-302, 2003

Jackson: "The lymphatics revisited: new perspectives from the hyaluronan receptor LYVE-1." in: Trends in cardiovascular medicine, Vol. 13, Issue 1, pp. 1-7, 2003

Fonsatti, Del Vecchio, Altomonte, Sigalotti, Nicotra, Coral, Natali, Maio: "Endoglin: An accessory component of the TGF-beta-binding receptor-complex with diagnostic, prognostic, and bioimmunotherapeutic potential in human malignancies." in: Journal of cellular physiology, Vol. 188, Issue 1, pp. 1-7, 2001

Mouta Carreira, Nasser, di Tomaso, Padera, Boucher, Tomarev, Jain: "LYVE-1 is not restricted to the lymph vessels: expression in normal liver blood sinusoids and down-regulation in human liver cancer and cirrhosis." in: Cancer research, Vol. 61, Issue 22, pp. 8079-84, 2001

Sleeman, Krishnan, Kirkin, Baumann: "Markers for the lymphatic endothelium: in search of the holy grail?" in: Microscopy research and technique, Vol. 55, Issue 2, pp. 61-9, 2001

Mäkinen, Veikkola, Mustjoki, Karpanen, Catimel, Nice, Wise, Mercer, Kowalski, Kerjaschki, Stacker, Achen, Alitalo: "Isolated lymphatic endothelial cells transduce growth, survival and migratory signals via the VEGF-C/D receptor VEGFR-3." in: The EMBO journal, Vol. 20, Issue 17, pp. 4762-73, 2001

Arthur, Ure, Smith, Renforth, Wilson, Torsney, Charlton, Parums, Jowett, Marchuk, Burn, Diamond: "Endoglin, an ancillary TGFbeta receptor, is required for extraembryonic angiogenesis and plays a key role in heart development." in: Developmental biology, Vol. 217, Issue 1, pp. 42-53, 2000

Barbara, Wrana, Letarte: "Endoglin is an accessory protein that interacts with the signaling receptor complex of multiple members of the transforming growth factor-beta superfamily." in: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 584-94, 1999

McAllister, Grogg, Johnson, Gallione, Baldwin, Jackson, Helmbold, Markel, McKinnon, Murrell: "Endoglin, a TGF-beta binding protein of endothelial cells, is the gene for hereditary haemorrhagic telangiectasia type 1." in: Nature genetics, Vol. 8, Issue 4, pp. 345-51, 1995

Cheifetz, Bellón, Calés, Vera, Bernabeu, Massagué, Letarte: "Endoglin is a component of the transforming growth factor-beta receptor system in human endothelial cells." in: The Journal of biological chemistry, Vol. 267, Issue 27, pp. 19027-30, 1992