MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (AA 76-87)

Details for Product anti-MAP3K5 Antibody No. ABIN116930, Supplier: Log in to see
Antigen
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAPKKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
  • MEKK5
  • MAP3K5
  • mitogen-activated protein kinase kinase kinase 5
  • Map3k5
  • MAPKKK5
  • MAP3K5
Alternatives
anti-Human MAP3K5 antibody for ELISA
Epitope
AA 76-87, pSer83
60
48
37
19
19
18
15
15
15
14
11
10
6
5
5
5
4
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
382
170
96
6
1
1
1
1
1
1
Host
Rabbit
362
26
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
12
12
11
6
6
6
5
5
5
5
5
5
5
5
5
5
5
Application
Western Blotting (WB)
287
146
127
81
65
41
20
10
10
10
4
3
2
1
1
Options
Supplier
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Immunogen This purified antibody was prepared from rabbit serum after repeated immunizations with a KLH conjugated peptide corresponding to amino acids 76-87 of human ASK-1 protein.
Specificity This product is an IgG fraction antibody purified from antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. No reaction was observed with ASK-1 from mouse sources. Reactivity with the kinase from other sources has not been determined.
Purification Multi-step process.
Alternative Name MEKK5 / ASK1 (MAP3K5 Antibody Abstract)
Background ASK-1 (apoptosis signal-regulating kinase 1- also referred to as MEK Kinase-5 or MAPKKK5) is a novel serine/threonine MAP kinase kinase kinase (MAPKKK) component of the mitogen -activated protein (MAP) cascade that is activated in response to extracellular stimuli by cytokines, growth factors and environmental stresses and other factors. Overexpression of ASK-1 induces apoptotic cell death. ASK-1 is expressed in a variety of human and mouse tissues. The overall amino acid sequence identity between the mouse and human ASK1 is 91.9 % . ASK-1 interacts with CDKN1A (also known as p21, WAF1, CIP1). Please refer to the reference list at the end of this document for further information.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5
Gene ID 4217
NCBI Accession NP_005914
UniProt Q99683
Pathways MAPK Signaling, Positive Regulation of Endopeptidase Activity
Application Notes This phospho specific polyclonal antibody reacts human pS83 ASK1 and shows minimalreactivity by Western blot, ELISA and competitive ELISA with non-phosphorylated ASK1. Although not tested, this antibody is likely functional in RIA, Immunohistochemistry andImmunoprecipitation. Recommended Dilutions: For immunoblotting a 1/1,000 dilution is recommended. A 155 kDa band corresponding to human ASK-1 is detected. Whole cell lysates from SW1353 canbe used as a positive control. For ELISA a 1/5,000-1/10,000 dilution is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 2.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use . Avoid cycles of freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (AA 76-87) (ABIN116930) Immunoblot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human AS...
 image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (AA 76-87) (ABIN116930) ELISA results of purified polyclonal anti-pS83 ASK-1 (aa 76-87) antibody tested again...
Background publications Matsuzawa, Ichijo: "Molecular mechanisms of the decision between life and death: regulation of apoptosis by apoptosis signal-regulating kinase 1." in: Journal of biochemistry, Vol. 130, Issue 1, pp. 1-8, 2001 (PubMed).

Noguchi, Kokubu, Kitanaka, Ichijo, Kuchino: "ASK1-signaling promotes c-Myc protein stability during apoptosis." in: Biochemical and biophysical research communications, Vol. 281, Issue 5, pp. 1313-20, 2001 (PubMed).

Tobiume, Matsuzawa, Takahashi, Nishitoh, Morita, Takeda, Minowa, Miyazono, Noda, Ichijo: "ASK1 is required for sustained activations of JNK/p38 MAP kinases and apoptosis." in: EMBO reports, Vol. 2, Issue 3, pp. 222-8, 2001 (PubMed).

Morita, Saitoh, Tobiume, Matsuura, Enomoto, Nishitoh, Ichijo: "Negative feedback regulation of ASK1 by protein phosphatase 5 (PP5) in response to oxidative stress." in: The EMBO journal, Vol. 20, Issue 21, pp. 6028-36, 2001 (PubMed).

Zou, Tsutsui, Ray, Blomquist, Ichijo, Ucker, Kiyokawa: "The cell cycle-regulatory CDC25A phosphatase inhibits apoptosis signal-regulating kinase 1." in: Molecular and cellular biology, Vol. 21, Issue 14, pp. 4818-28, 2001 (PubMed).

Geleziunas, Xu, Takeda, Ichijo, Greene: "HIV-1 Nef inhibits ASK1-dependent death signalling providing a potential mechanism for protecting the infected host cell." in: Nature, Vol. 410, Issue 6830, pp. 834-8, 2001 (PubMed).

Sayama, Hanakawa, Shirakata, Yamasaki, Sawada, Sun, Yamanishi, Ichijo, Hashimoto: "Apoptosis signal-regulating kinase 1 (ASK1) is an intracellular inducer of keratinocyte differentiation." in: The Journal of biological chemistry, Vol. 276, Issue 2, pp. 999-1004, 2001 (PubMed).

Cho, Lee, Park, Ryoo, Kang, Park, Eom, Kim, Chang, Choi, Shim, Kim, Dong, Lee, Kim, Ichijo, Choi: "Glutathione S-transferase mu modulates the stress-activated signals by suppressing apoptosis signal-regulating kinase 1." in: The Journal of biological chemistry, Vol. 276, Issue 16, pp. 12749-55, 2001 (PubMed).

Liu, Nishitoh, Ichijo, Kyriakis: "Activation of apoptosis signal-regulating kinase 1 (ASK1) by tumor necrosis factor receptor-associated factor 2 requires prior dissociation of the ASK1 inhibitor thioredoxin." in: Molecular and cellular biology, Vol. 20, Issue 6, pp. 2198-208, 2000 (PubMed).

Kanamoto, Mota, Takeda, Rubin, Miyazono, Ichijo, Bazenet: "Role of apoptosis signal-regulating kinase in regulation of the c-Jun N-terminal kinase pathway and apoptosis in sympathetic neurons." in: Molecular and cellular biology, Vol. 20, Issue 1, pp. 196-204, 2000 (PubMed).

Yasuda, Inoue, Hirabayashi, Higashiyama, Yamamoto, Fuyuhiro, Komure, Tanaka, Sobue, Tsuchiya, Hamada, Sasaki, Takeda, Ichijo, Kakizuka: "Triggering of neuronal cell death by accumulation of activated SEK1 on nuclear polyglutamine aggregations in PML bodies." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 4, Issue 12, pp. 743-56, 2000 (PubMed).

Takeda, Hatai, Hamazaki, Nishitoh, Saitoh, Ichijo: "Apoptosis signal-regulating kinase 1 (ASK1) induces neuronal differentiation and survival of PC12 cells." in: The Journal of biological chemistry, Vol. 275, Issue 13, pp. 9805-13, 2000 (PubMed).

Hatai, Matsuzawa, Inoshita, Mochida, Kuroda, Sakamaki, Kuida, Yonehara, Ichijo, Takeda: "Execution of apoptosis signal-regulating kinase 1 (ASK1)-induced apoptosis by the mitochondria-dependent caspase activation." in: The Journal of biological chemistry, Vol. 275, Issue 34, pp. 26576-81, 2000 (PubMed).

Mochida, Takeda, Saitoh, Nishitoh, Amagasa, Ninomiya-Tsuji, Matsumoto, Ichijo: "ASK1 inhibits interleukin-1-induced NF-kappa B activity through disruption of TRAF6-TAK1 interaction." in: The Journal of biological chemistry, Vol. 275, Issue 42, pp. 32747-52, 2000 (PubMed).