His Tag antibody

Details for Product No. ABIN116941, Supplier: Log in to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
616
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2
Host
Rabbit
435
183
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23
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1
Clonality
Polyclonal
Conjugate
Un-conjugated
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45
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1
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1
1
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1
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1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
554
386
167
107
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25
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2
1
1
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Options
Supplier
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Supplier Product No.
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Immunogen 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide
Sequence HHHHHH
Purification Affinity Chromatography.
Alternative Name 6xHistidine Epitope Tag
Target Type Tag
Background Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.
Application Notes Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. This antibody has been tested by ELISA and western blotting against both the immunizingpeptide and His-containing recombinant proteins. Although not tested, this antibody islikely functional for Immunoprecipitation and Immunocytochemistry. Recommended Dilutions: ELISA: 1/10,000-1/40,000. Western blot: 1/1,000-1/3,000. Immunohistochemistry: 1/500-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage -20 °C
Storage Comment Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.
Supplier Images
Western Blotting (WB) image for anti-His Tag antibody (ABIN116941) Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins b...
Background publications Yi, Ba, Zhang, Ho, Chen: "Single amino acid substitutions in the severe acute respiratory syndrome coronavirus spike glycoprotein determine viral entry and immunogenicity of a major neutralizing domain." in: Journal of virology, Vol. 79, Issue 18, pp. 11638-46, 2005 (PubMed).

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