IL-18 antibody

Catalog No. ABIN116946

Product Details anti-IL-18 Antibody

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-18 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody is primarily directed against mature 18,000 MW mouse IL-18 and is useful in determining its presence in various assays. This antibody will also recognize the 24,000 inactive precursor form of Mouse IL-18. In general, this antibody also detects rat IL-18 in the same formats using similar dilutions.

Purification: Protein A chromatography

Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with native 157 aa mouse IL-18 produced in E.coli.

Application Details

Application Notes: This antibody is suitable for use in Neutralizations, ELISA, Immunohistochemistry andImmunoblotting. Although untested, this reagent may be useful for Radioimmunoassays,Flow cytometry and Immunoprecipitation. It recognizes the 18,000 MW mature (active)IL-18. Reactivity in other Immunoassays is unknown. Recommended Dilutions: This product has been assayed by immunoblot using HRP

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing no stabilizers or preservatives

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

References for anti-IL-18 antibody (ABIN116946)

Sakai, Sugawara, Kuroishi, Sasano, Sugawara: "Identification of IL-18 and Th17 cells in salivary glands of patients with Sjögren's syndrome, and amplification of IL-17-mediated secretion of inflammatory cytokines from salivary gland cells by IL-18." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 4, pp. 2898-906, (2008) (PubMed).

Lauritsen, Madsen: "Synthesis of naturally occurring iminosugars from D-fructose by the use of a zinc-mediated fragmentation reaction." in: Organic & biomolecular chemistry, Vol. 4, Issue 15, pp. 2898-905, (2006) (PubMed).

Target Details for IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: Interleukin-18 / IL18 (IL18 Products)

Synonyms: Igif antibody, Il-18 antibody, IL-18 antibody, IGIF antibody, ChIL-18 antibody, IL-1g antibody, IL1F4 antibody, IL18 antibody, interleukin 18 antibody, Il18 antibody, IL18 antibody

Background: Interleukin-18 (IL-18) is a member of the IL-1 cytokine family and was initially identified as an Interferon-g (IFN-g) inducing factor (IGIF). The IL-18 gene was originally cloned from liver cells and has since been shown to be produced by activated monocytes/ macrophages, Kupffer cells, keratinocytes, glucocorticoid-secreting adrenal cortex cells, osteoblasts and dendritic cells. IL-18 is a 24 kDa, non-glycosylated polypeptide that lacks a classical signal sequence and possesses a structure recognizably similar to IL-1. IL-18 is synthesized as a bio-inactive propeptide that undergoes proteolytic cleavage by either ICE (interleukin-1 beta converting enzyme) or another caspase to generate a mature, bioactive, 18 kDa molecule. In both the mature and propeptide forms, IL-18 shows 64 % aa sequence identity from mouse to human. IL-18 does not appear to show any primary sequence similarity to any other known cytokines. Rat IL-18 has also been isolated, and found to be 194 aa in length with a 91 % aa sequence identity to mouse IL-18. Human IL-18 has been found to induce the production of IFN-g and GM-CSF while inhibiting the production of IL-10 by PBMC. With respect to human T cells, IL-18 enhances Th1 cytokine production and stimulates cell proliferation via an IL-2-dependent pathway. Human IL-18 can also inhibit the synthesis of IgE by B cells. Thus, IL-18 plays an important role in immunological and inflammatory reactions. Currently, the bioactivity of human IL-18 is often determined by its capacity to augment the levels of IFN-g produced by T cells as measured in tissue culture supernatants.Synonyms: IGIF, IL-1 gamma, IL-18, IL1 gamma, IL1F4, Iboctadekin, Interferon-gamma-inducing factor, Interleukin-1 gamma

Gene ID: 16173

NCBI Accession: NP_032386

UniProt: P70380

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome