IL-6 antibody

Catalog No. ABIN1176903

Product Details anti-IL-6 Antibody

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-6 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Neutralization (Neut)

Brand: BD Pharmingen™

Characteristics: Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human IL-6. Neutralization activity may be measured with a proliferation assay using 500 pg/mL recombinant human IL-6 (Cat. No. 550071) to stimulate TF-1 cells at 1x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant human IL-6 for 60 minutes).
50% Neutralization (ND50) at 0.2 - 1.0 ng/mL
≥ 95 % Neutralization at 1.5 - 4.5 ng/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Human IL-6 Recombinant Protein

Clone: MQ2-13A5

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL-6 antibody (ABIN1176903)

Gaines Das, Poole: "The international standard for interleukin-6. Evaluation in an international collaborative study." in: Journal of immunological methods, Vol. 160, Issue 2, pp. 147-53, (1993) (PubMed).

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6 (IL6 Products)

Synonyms: BSF2 antibody, HGF antibody, HSF antibody, IFNB2 antibody, IL-6 antibody, Il-6 antibody, ILg6 antibody, Ifnb2 antibody, il6 antibody, CHIL-6 antibody, interleukin 6 antibody, interleukin-6 antibody, IL6 antibody, Il6 antibody, il-6 antibody, IL-6 antibody

Background: The MQ2-13A5 monoclonal antibody specifically binds to human interleukin-6 (IL-6). The immunogen used to generate this hybridoma was COS-7 -expressed recombinant human IL-6.
Synonyms: IL6, Interleukin-6, BSF-2, CDF, HGF, HSF, IFNB2

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome