GSK3 alpha antibody (pSer21)

Catalog No. ABIN117908

There is 1 publication for this product available. The Rabbit Polyclonal anti-GSK3 alpha antibody is suitable to detect GSK3 alpha in samples from Human. It has been validated for WB and EIA.

Quick Overview for GSK3 alpha antibody (pSer21) (ABIN117908)

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GSK3 alpha antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-GSK3 alpha Antibody

Binding Specificity: AA 16-25, pSer21

Sequence: Protein Sequence: Human GSK3 alpha, 483 aa, predicted MW 51.0 kDa 1 msgggpsggg pggsgrarts sfaepggggg gggggpggsa sgpggtgggk asvgamgggv 61 gasssgggpg gsggggsggp gagtsfpppg vklgrdsgkv ttvvatlgqg persqevayt 121 dikvigngsf gvvyqarlae trelvaikkv lqdkrfknre lqimrkldhc nivrlryffy 181 ssgekkdely lnlvleyvpe tvyrvarhft kakltipily vkvymyqlfr slayihsqgv 241 chrdikpqnl lvdpdtavlk lcdfgsakql vrgepnvsyi csryyrapel ifgatdytss 301 idvwsagcvl aelllgqpif pgdsgvdqlv eiikvlgtpt reqiremnpn ytefkfpqik 361 ahpwtkvfks rtppeaialc sslleytpss rlspleacah sffdelrclg tqlpnnrplp 421 plfnfsagel siqpslnail ipphlrspag tttltpssqa ltetptssdw qstdatptlt 481 nss

Specificity: This affinity-purified antibody is directed against the phosphorylated form of human GSK3A at the pS 21 residue. The product was affinity purified from monospecific antiserum by Immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody reacts with phosphorylated pS21 of human GSK3A. Reactivity with non-phosphorylated human GSK3A is minimal. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and rat based on 100 % homology for the immunogen sequence. Cross reactivity with GSK3A homologues from other sources has not been determined.

Purification: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 16-25 of human GSK3 alpha.

Application Details

Application Notes: This affinity-purified antibody has been tested for use in ELISA (1: 2,000-1: 10,000) and byWestern blot (1: 500-1: 3,000). Specific conditions for reactivity should be optimized by theend user. Expect a band approximately 52 kDa in size corresponding to GSK3A by Westernblotting in the appropriate cell lysate or extract. Less than 1 % reactivity is observed against the non-phosphorylated form of theimmunizing peptide. This antibody is phosphor specific for pS21 of GSK3A.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-GSK3 alpha antibody (ABIN117908)

Wang, He, Wang, Wang, Zhai, Zhou, Zhang, Zhai, Feng, Han: "[Impact of Donor KIR2DS5 Genotype on Outcome Following Haploidentical Hematopoietic Stem Cell Transplantation.]" in: Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology, Vol. 16, Issue 1, pp. 111-5, (2008) (PubMed).

Target Details for GSK3 alpha

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Alternative Name: GSK3 alpha

Background: Glycogen synthase kinase 3 alpha (GSK3A) belongs to the ser/thr family of protein kinases, Cdc2/cdkx subfamily, gsk-3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c-jun. GSK3A is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A) and beta (GSK3B), show a high degree of amino acid homology. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.Synonyms: Factor A, GSK-3 alpha, GSK3A, Glycogen synthase kinase-3 alpha

Gene ID: 2931

NCBI Accession: NP_063937

UniProt: P49840

Pathways: PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effect