CD47 antibody

Catalog No. ABIN118685

The Mouse Monoclonal anti-CD47 antibody has been validated for FACS, IP and IHC (fro). It is suitable to detect CD47 in samples from Human, Pig, Cow, Dog and Sheep.

Quick Overview for CD47 antibody (ABIN118685)

Target: CD47

Reactivity: Human, Pig, Cow, Dog, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD47 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: BRIC-126

Product Details anti-CD47 Antibody

Specificity: This antibody recognises the CD47 cell surface glycoprotein.

Purification: Affinity Chromatography on Protein G.

Immunogen: Human erythrocytes.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/10 diluted antibody to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD47

Target: CD47

Alternative Name: CD47

Background: CD47 is a heavily N-glycosylated 47-52kD molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype.Synonyms: Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, OA3

Gene ID: 961

NCBI Accession: NP_001768

UniProt: Q08722