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ICAM-1 antibody (Intercellular Adhesion Molecule 1)

Details for Product anti-ICAM1 Antibody No. ABIN118732, Supplier: Log in to see
Antigen
  • BB2
  • CD54
  • ICAM
  • ICAM-1
  • Icam-1
  • ICAM1
  • Ly-47
  • MALA-2
  • P3.58
Reactivity
Dog (Canine), Human
738
170
149
26
8
6
1
1
1
Host
Mouse
578
258
87
10
9
5
4
2
Clonality (Clone)
Monoclonal ()
Conjugate
This ICAM-1 antibody is un-conjugated
85
82
80
20
19
19
13
11
9
9
9
9
8
8
8
8
8
6
5
5
5
4
4
4
4
4
4
3
3
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
570
379
235
206
196
152
145
69
51
49
35
29
20
13
12
12
10
9
8
6
6
6
4
3
3
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Immunogen K562 cell line. Spleen cells from immunised BALB/c mice were fused with cells of the MOPC 315 mouse myeloma cell line.
Clone 84H10
Isotype IgG1
Purification Affinity Chromatography on Protein A
Alternative Name CD54 / ICAM1 (ICAM1 Antibody Abstract)
Background ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.Synonyms: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor
Gene ID 9606
UniProt P05362
Application Notes Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections (1/20-1/50). Immunoprecipitation: 5 μg/10e7 cells. Removal of sodium azide is recommended prior to use in functional assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Galkowska, Waldemar, Wojewodzka: "Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes." in: Veterinary immunology and immunopathology, Vol. 53, Issue 3-4, pp. 329-34, 1997 (PubMed).

Berendt, McDowall, Craig, Bates, Sternberg, Marsh, Newbold, Hogg: "The binding site on ICAM-1 for Plasmodium falciparum-infected erythrocytes overlaps, but is distinct from, the LFA-1-binding site." in: Cell, Vol. 68, Issue 1, pp. 71-81, 1992 (PubMed).

Simmons, Makgoba, Seed: "ICAM, an adhesion ligand of LFA-1, is homologous to the neural cell adhesion molecule NCAM." in: Nature, Vol. 331, Issue 6157, pp. 624-7, 1988 (PubMed).

Makgoba, Sanders, Ginther Luce, Dustin, Springer, Clark, Mannoni, Shaw: "ICAM-1 a ligand for LFA-1-dependent adhesion of B, T and myeloid cells." in: Nature, Vol. 331, Issue 6151, pp. 86-8, 1988 (PubMed).

Dustin, Rothlein, Bhan, Dinarello, Springer: "Induction by IL 1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 1, pp. 245-54, 1986 (PubMed).

Rothlein, Dustin, Marlin, Springer: "A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 4, pp. 1270-4, 1986 (PubMed).