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HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody

Details for Product No. ABIN119228, Supplier: Log in to see
Antigen
  • MHC-G
  • B-F
  • B-F-S04
  • B-F-S05
  • B-F-S06
  • B-F-S07
  • B-FI
  • B-FIV
  • BF2
  • BFa2
  • BFw-03
  • BFw-05
  • BFz-01
  • major histocompatibility complex, class I, G
  • MHC BF1 class I
  • HLA-G
  • BF1
Alternatives
anti-Human HLA Class I Histocompatibility Antigen, alpha Chain G antibody for ELISA
Reactivity
Human
506
41
9
8
1
1
Host
Mouse
410
72
26
6
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
52
50
36
28
12
9
9
9
9
9
9
9
9
9
9
8
8
8
7
5
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
300
292
282
156
132
129
118
111
78
23
21
14
8
7
5
3
3
2
2
1
1
1
1
Options
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Immunogen Recombinant Human HLA-G denaturated heavy chain
Clone MEM-G-1
Isotype IgG1
Specificity The antibody reacts with denaturated HLA-G heavy chain. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.
Purification Affinity Chromatography on Protein-A
Alternative Name HLA Class I alpha G / HLA-G (HLAG Antibody Abstract)
Background HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.Synonyms: HLA class I histocompatibility antigen, HLA-6.0, HLAG, MHC class I antigen G
Gene ID 3135
NCBI Accession NP_002118
UniProt P17693
Pathways Regulation of Leukocyte Mediated Immunity
Application Notes Western Blotting. Immunohistochemistry (paraffin sections): 1:60 to 1:100, 1 h at RT. Positive tissue: Human placenta - extravillous cytotrophoblast. Staining technique: Standard ABC technique. Pretreatment: Heat retrieval in 0.01 M citrate buffer (4x2 min. in microwave oven).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to - 70 °C for longer.
Background publications Gonen-Gross, Achdout, Arnon, Gazit, Stern, Horejsí, Goldman-Wohl, Yagel, Mandelboim: "The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 4866-74, 2005 (PubMed).

Shih, Kurman: "p63 expression is useful in the distinction of epithelioid trophoblastic and placental site trophoblastic tumors by profiling trophoblastic subpopulations." in: The American journal of surgical pathology, Vol. 28, Issue 9, pp. 1177-83, 2004 (PubMed).

Boyson, Erskine, Whitman, Chiu, Lau, Koopman, Valter, Angelisova, Horejsi, Strominger: "Disulfide bond-mediated dimerization of HLA-G on the cell surface." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 25, pp. 16180-5, 2002 (PubMed).

Hurks, Valter, Wilson, Hilgert, van den Elsen, Jager: "Uveal melanoma: no expression of HLA-G." in: Investigative ophthalmology & visual science, Vol. 42, Issue 13, pp. 3081-4, 2001 (PubMed).

Fournel, Huc, Aguerre-Girr, Solier, Legros, Praud-Brethenou, Moussa, Chaouat, Berrebi, Bensussan, Lenfant, Le Bouteiller: "Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules." in: Tissue antigens, Vol. 55, Issue 6, pp. 510-8, 2000 (PubMed).

Frumento, Franchello, Palmisano, Nicotra, Giacomini, Loke, Geraghty, Maio, Manzo, Natali, Ferrara: "Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment." in: Tissue antigens, Vol. 56, Issue 1, pp. 30-7, 2000 (PubMed).