His Tag antibody (Biotin)

Details for Product No. ABIN119351, Supplier: Log in to see
Antigen
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
Reactivity
Tag
616
18
3
1
Host
Mouse
397
178
27
23
4
4
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
58
43
35
15
13
11
9
8
8
7
7
7
7
7
7
7
6
6
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
528
372
159
106
77
75
66
25
12
10
9
7
7
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Options
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Available images

Immunogen PAX6 transcription factor linked to histidine tag
Sequence HHHHHH
Clone AD1-1-10
Isotype IgG1
Purification Protein G affinity chromatography
Alternative Name 6xHistidine Epitope Tag
Target Type Tag
Application Notes ELISA: 1/1000. Western Blot: 1/1000. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product is routinely tested in ELISA using histidine tagged ubiquitin and in Western blotting using histidine tagged recombinant protein control.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS with 0.09 % sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-His Tag antibody (Biotin) (ABIN119351) Western blot of HIS tagged protein probed with mouse anti Histidine Tag - Biotin (ABI...
Background publications Bahi, Boyer, Kafri, Dreyer: "CD81-induced behavioural changes during chronic cocaine administration: in vivo gene delivery with regulatable lentivirus." in: The European journal of neuroscience, Vol. 19, Issue 6, pp. 1621-33, 2004 (PubMed).

Els Conrath, Lauwereys, Wyns, Muyldermans: "Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7346-50, 2001 (PubMed).