CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C) (FITC)

Details for Product anti-PTPRC Antibody No. ABIN120462, Supplier: Log in to see
Antigen
  • PTPRC
  • si:dkeyp-47c5.1
  • B220
  • CD45
  • CD45R
  • GP180
  • L-CA
  • LCA
  • LY5
  • T200
  • Cd45
  • Ly-5
  • Lyt-4
  • loc
  • Lca
  • RT7
  • cd45
  • protein tyrosine phosphatase, receptor type, C
  • PTPRC
  • ptprc
  • Ptprc
Alternatives
anti-Rat (Rattus) CD45 antibody for Cytometry by Time of Flight
Reactivity
Rat (Rattus)
1358
713
184
128
65
45
45
43
23
20
19
12
6
6
4
3
2
1
1
1
1
1
1
Host
Mouse
1377
737
235
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD45 antibody is conjugated to FITC
251
222
167
91
66
62
61
54
48
41
36
34
33
33
33
29
28
26
26
21
19
17
14
14
13
13
12
11
10
10
9
7
6
6
6
5
5
5
5
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1932
536
531
521
464
436
409
361
202
54
38
31
28
16
11
10
7
7
6
6
4
3
3
2
2
2
2
2
2
2
1
1
1
Options
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Immunogen Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Clone OX-1
Isotype IgG1
Specificity This antibody recognises CD45. Antibodies recognising a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes.
Alternative Name CD45 / LCA (PTPRC Antibody Abstract)
Background The leucocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180 - 240 kDa. CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Gene ID 24699
NCBI Accession NP_001103357
UniProt P04157
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events
Application Notes Flow Cytometry: Use 10 μL of 1/50 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Sato, Balla, Otterbein, Smith, Brouard, Lin, Csizmadia, Sevigny, Robson, Vercellotti, Choi, Bach, Soares: "Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 6, pp. 4185-94, 2001 (PubMed).

Murakami, Sato, Nagasawa, Yamashita: "Regulation of mast cell signaling through high-affinity IgE receptor by CD45 protein tyrosine phosphatase." in: International immunology, Vol. 12, Issue 2, pp. 169-76, 2000 (PubMed).

Martín, Molina, Bricio, Mampaso: "Passive dual immunization against tumour necrosis factor-alpha (TNF-alpha) and IL-1 beta maximally ameliorates acute aminonucleoside nephrosis." in: Clinical and experimental immunology, Vol. 99, Issue 2, pp. 283-8, 1995 (PubMed).

Barclay, Jackson, Willis, Williams: "Lymphocyte specific heterogeneity in the rat leucocyte common antigen (T200) is due to differences in polypeptide sequences near the NH2-terminus." in: The EMBO journal, Vol. 6, Issue 5, pp. 1259-64, 1987 (PubMed).

Woollett, Barclay, Puklavec, Williams: "Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes." in: European journal of immunology, Vol. 15, Issue 2, pp. 168-73, 1985 (PubMed).

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981 (PubMed).

Sunderland, McMaster, Williams: "Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes." in: European journal of immunology, Vol. 9, Issue 2, pp. 155-9, 1979 (PubMed).