LAT antibody

Catalog No. ABIN120717

Product Details anti-LAT Antibody

Target: LAT (Linker For Activation of T Cells (LAT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAT antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)

Specificity: The antibody reacts with Linker for activation of T cells (LAT), a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. Does not react with mouse.

Characteristics: Synonyms: pp36, p36-38, 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-CellsFamily Member 1

Purification: Protein A affinity chromatography

Purity: > 95 % (by SDS-PAGE)

Immunogen: Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmicdomain of human LAT

Clone: LAT-01

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Western Blot (Tyrosine-phosphorylated form of LAT is not optimally recognized). Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for LAT

Target: LAT (Linker For Activation of T Cells (LAT))

Alternative Name: LAT (LAT Products)

Synonyms: LAT antibody, lat antibody, LAT1 antibody, pp36 antibody, linker for activation of T-cells antibody, linker for activation of T cells antibody, LAT antibody, lat antibody, Lat antibody

Background: LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FceRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.Synonyms: 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-Cells Family Member 1, p36-38, pp36

Gene ID: 27040

UniProt: O43561

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway