MIF antibody (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor)) (AA 1-114)

Details for Product anti-MIF Antibody No. ABIN121160, Supplier: Log in to see
Antigen
  • mif
  • Mif
  • gif
  • glif
  • mmif
  • LOC100136498
  • LOC100284350
  • LOC100284546
  • GIF
  • Glif
  • GLIF
  • MMIF
  • macrophage migration inhibitory factor (glycosylation-inhibiting factor)
  • macrophage migration inhibitory factor
  • Macrophage migration inhibitory factor
  • mif
  • MIF
  • Mif
  • PHATRDRAFT_49660
  • LOC100136498
  • LOC100284350
  • LOC100284546
  • LOC100338701
Alternatives
anti-Human MIF antibody for Flow Cytometry
Epitope
AA 1-114, N-Term
37
30
28
13
10
8
4
3
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human
194
74
73
7
6
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4
3
2
2
2
1
1
Host
Mouse
110
69
31
24
4
1
Clonality (Clone)
Monoclonal ()
Conjugate
This MIF antibody is un-conjugated
18
10
6
6
6
6
6
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5
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2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
176
113
51
42
29
23
13
11
10
6
5
4
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Recombinant human MIF (1-114 aa) purified from E. coli
Clone 4-00E-004
Isotype IgG1
Specificity The antibody recognizes Human MIF. Other species not tested.
Purification Protein-G affinity chromatography
Alternative Name MIF (MIF Antibody Abstract)
Background The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T-cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.Synonyms: GLIF, Glycosylation-inhibiting factor, MMIF, Macrophage migration inhibitory factor, Phenylpyruvate tautomerase
Gene ID 4282
UniProt P14174
Research Area Immunology, Innate Immunity, Cytokines, Inflammation
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
Application Notes ELISA. Western blot (1/500 - 1/2,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, containing 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 1-114), (N-Term) antibody (ABIN121160) Western blot analysis.,.The extract of HL-60 was resolved by SDS-PAGE, transferred to...
Background publications Richard, Amador Del Valle, Moreau, Milpied, Felice, Daeschler, Harousseau, Richet: "Viridans streptococcal bacteraemia in patients with neutropenia." in: Lancet, Vol. 345, Issue 8965, pp. 1607-9, 1995 (PubMed).

Bernhagen, Mitchell, Calandra, Voelter, Cerami, Bucala: "Purification, bioactivity, and secondary structure analysis of mouse and human macrophage migration inhibitory factor (MIF)." in: Biochemistry, Vol. 33, Issue 47, pp. 14144-55, 1994 (PubMed).

Weiser, Temple, Witek-Giannotti, Remold, Clark, David: "Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 19, pp. 7522-6, 1989 (PubMed).

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