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Caspase 1 antibody (CASP1) (C-Term)

Details for Product anti-CASP1 Antibody No. ABIN121891, Supplier: Log in to see
Antigen
  • CASP1
  • ccp1
  • CG5370
  • dcp-1
  • DCP-1
  • dcp1
  • DCP1
  • Dcp1
  • Dmel\\CG5370
  • ICE
  • Ice
  • Il1bc
  • IL1BC
  • l(2)01862
  • l(2)02132
  • P45
Alternatives
anti-Human Caspase 1 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
C-Term
37
15
14
11
10
10
6
5
5
5
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
217
49
30
8
2
2
1
1
1
1
1
1
Host
Rabbit
222
17
10
2
Clonality
Polyclonal
Conjugate
This Caspase 1 antibody is un-conjugated
18
14
7
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
197
118
101
48
22
14
11
6
6
5
3
2
Supplier
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Immunogen A 16 amino acid peptide located near human Caspase-1 carboxy terminus
Specificity This antibody recognises an epitope within the C-terminal region (CT) of Caspase-1, otherwise known as IL-1Beta converting enzyme. This antibody detects a cleaved subunit band of approximately 21 kDa in heart cell lysates (predicted precursor MWT 45.2 kDa).
Purification Affinity chromatography
Alternative Name Caspase-1 (CASP1 Antibody Abstract)
Background Caspase-1 is an intracellular cysteine protease, identified as a mammalian homologue to C. elegans cell death gene (ced-3). Caspase-1 has been classified as an inflammatory, rather than apoptotic caspase, due to its essential role in the cleavage of the inactive precursors of the cytokines IL-1beta and IL-18, into their mature activated and secretable forms. This regulation of pro-inflammatory cytokines by Caspase-1 has made inhibitors of Caspase-1 a possible target for use as therapeutic drugs for the treatment of inflammatory diseases.Synonyms: CASP-1, CASP1, ICE, IL1BC, IL1BCE, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45
UniProt P29466
Research Area Apoptosis/Necrosis
Pathways Apoptosis
Application Notes Western Blot: 0.5 μg - 1.0 μg/mL. Immunohistochemistry on paraffin sections: 0.5 μg - 1.0 μg/mL, This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. Recommended Positive Control: Human heart tissue.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Expiry Date 12 months
Background publications Fantuzzi, Puren, Harding, Livingston, Dinarello: "Interleukin-18 regulation of interferon gamma production and cell proliferation as shown in interleukin-1beta-converting enzyme (caspase-1)-deficient mice." in: Blood, Vol. 91, Issue 6, pp. 2118-25, 1998

Ghayur, Banerjee, Hugunin, Butler, Herzog, Carter, Quintal, Sekut, Talanian, Paskind, Wong, Kamen, Tracey, Allen: "Caspase-1 processes IFN-gamma-inducing factor and regulates LPS-induced IFN-gamma production." in: Nature, Vol. 386, Issue 6625, pp. 619-23, 1997

Miura, Zhu, Rotello, Hartwieg, Yuan: "Induction of apoptosis in fibroblasts by IL-1 beta-converting enzyme, a mammalian homolog of the C. elegans cell death gene ced-3." in: Cell, Vol. 75, Issue 4, pp. 653-60, 1993

Kostura, Tocci, Limjuco, Chin, Cameron, Hillman, Chartrain, Schmidt: "Identification of a monocyte specific pre-interleukin 1 beta convertase activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 14, pp. 5227-31, 1989