HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody

Antigen: HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG)

Synonyms: MHC-G

Clonality: Monoclonal (MEM-G-9)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunocytochemistry (ICC)

Catalog no.: ABIN125707

Additional Information

Alternative name: HLA-G

Immunogen: Recombinant human HLA-G refolded with beta2-microglobulin and peptide.

Format: Azide Free

Isotype: IgG1

Clone: MEM-G-9

Specificity: The antibody MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as wellas with soluble HLA-G5 isoform in its beta2-microglobulin associated form. HLA-G belongsto the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on thesurface of trophoblast cells.The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd InternationalConference on HLA-G (Paris, 2003). This antibody does not react with: Mouse.

Application Details

Application Notes: Flow Cytometry: Recommended dilution: 1-5 µg/ml. Positive control: JEG-3 human choriocarcinoma cell line. Immunocytochemistry - Recommended dilution: Alexa Fluor® 488 Fab-fragment: 5 µg/ml. ELISA. Application note: The antibody MEM-G/9 has been tested as the capture antibody in asandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or withantibody W6/32. Coating antibody (10 µg/ml) Detection antibody (biotin or peroxidase conjugate, 1 µg/ml)

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by precipitation methods.

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Major Histocompatibility Complex (MHC)

Restrictions: For Research Use only