HLA-DR-gamma (CD74) antibody
| Antigen |
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| Synonyms |
DHLAG, HLADG, Ia-GAMMA, Ii, CLIP |
| Clonality |
Monoclonal (MEM-12) |
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Host
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Reactivity
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Human (119), Rat (Rattus) (21), Mouse (Murine) (20), Pig (Porcine) (18), Cow (Bovine) (17), Horse (Equine) (17), Dog (Canine) (16), Sheep (Ovine) (15), Baboon (2), Rabbit (2), Chicken (1)
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Application
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 Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
Flow Cytometry (FACS) (94), Immunoprecipitation (IP) (45), Western Blotting (WB) (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (30), Immunofluorescence (IF) (27), Immunohistochemistry (Frozen Sections) (IHC (fro)) (14), Immunohistochemistry (IHC) (8), Immunocytochemistry (ICC) (5), ELISA (3), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN125741 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
273.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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HLA-DR
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Immunogen
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thymocyte membrane
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Format
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Purified
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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MEM-12
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Specificity
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The antibody MEM-12 recognizes common epitope on human HLA-DR which is dependenton the association of alpha and beta chains. DR is the isotype of human MHC Class IImolecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes,monocytes, macrophages).
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Application Notes
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Flow Cytometry - Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line. Immunoprecipitation - Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line. - Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing SDS-PAGE sample buffer. Do not heat/boil. Application note: Non-reducing conditions.
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from ascites by protein-A affinity chromatography.
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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Major Histocompatibility Complex (MHC), CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Publications
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Simpson, McFarlin, McSporran et al.: "Toll-like receptor expression on classic and pro-inflammatory blood monocytes after acute exercise in humans." in: Brain, behavior, and immunity, Vol. 23, Issue 2, pp. 232-9, 2009 (PubMed).
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Alternatives for antigen "HLA-DR-gamma (CD74)", type "Antibodies"
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Hosts
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Mouse (91), Rabbit (23), Rat (8)
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Reactivities
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Human (119), Rat (Rattus) (21), Mouse (Murine) (20), Pig (Porcine) (18), Cow (Bovine) (17), Horse (Equine) (17), Dog (Canine) (16), Sheep (Ovine) (15), Baboon (2), Rabbit (2), Chicken (1)
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Applications
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Flow Cytometry (FACS) (94), Immunoprecipitation (IP) (45), Western Blotting (WB) (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (30), Immunofluorescence (IF) (27), Immunohistochemistry (Frozen Sections) (IHC (fro)) (14), Immunohistochemistry (IHC) (8), Immunocytochemistry (ICC) (5), ELISA (3), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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FITC (13), PE (9), Biotin (7), APC (5), Alexa Fluor 488 (2), Alexa Fluor 700 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), DY647 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), Pacific Blue (1), PerCP (1), RBITC (1), RPE (1)
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