HLA-DR antibody (HLA-DR)

Details for Product anti-HLA-DR Antibody No. ABIN94340
Antigen
Reactivity
Human
323
29
21
13
10
7
6
5
4
4
3
3
2
1
1
1
1
1
Host
Mouse
261
47
17
Clonality (Clone)
Monoclonal ()
Conjugate
This HLA-DR antibody is un-conjugated
49
49
24
18
13
11
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
278
65
63
47
39
30
25
17
12
9
7
6
6
6
3
1
1
1
1
Options
Immunogen Raji Burkitt's lymphoma cell line
Clone HL-39
Isotype IgG3
Specificity The antibody HL-39 recognizes common epitope on human HLA-DR which is dependent on the association of alpha and beta chains. Recognized epitope is conserved after fixation with glutaraldehyde and formaldehyde. DR is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name HLA-DR
Background HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, 2011 (PubMed).

Horejsí, N?mec, Angelisová, Kristofová, Gorga, Hilgert: "Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens." in: Tissue antigens, Vol. 28, Issue 5, pp. 288-97, 1987 (PubMed).