RIP antibody

Antigen: RIP

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN127073

Additional Information

Alternative name:

UniProt: Q13546

Immunogen: Synthetic peptide surrounding amino acid 645 of Human RIP

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: RIP (Receptor Interacting Protein) is a 74 kDa Ser/Thr kinase which interacts with CD95(Fas/Apo1) receptor and the tumor necrosis factor receptor (TNFR1). It is a cell deathdomain adapter protein which can bind to the adapter proteins TRADD, RAID, and TRAF2. RIP contains an N-terminal region with homology to protein kinase, an intermediatedomain capable of association with MAPKKK and a C-terminal region containing anintracellular death domain motif. RIP activates both p38 MAP kinase and SAPK families. Invitro, RIP induces apoptosis, as well as SAPK/JNK and NF-kB activation. RIP possesseskinase activity as it autophosphorylates itself on serine and threonine residues.

Characteristics: Synonyms: RIPK1, Receptor-interacting serine/threonine-protein kinase 1, Serine/threonine-proteinkinase RIP, Cell death protein RIP, Receptor-Interacting Protein

Application Details

Application Notes: Western Blot: 2-6 µg/ml. Immunoprecipitation. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Affinity Purified

Buffer: PBS, pH 7.2, containing 30% Glycerol, 0.5% BSA as stabilizer and 0.01%Thimerosal as preservative.

Storage: Store the antibody undiluted at -20°C or (in aliquots) at -70°C for long term storage. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Cancer, Apoptosis/Necrosis

Restrictions: For Research Use only

Publications

Stanger, Leder, Lee et al.: "RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death." in: Cell, Vol. 81, Issue 4, pp. 513-23, 1995 (PubMed).

Hsu, Huang, Shu et al.: "TNF-dependent recruitment of the protein kinase RIP to the TNF receptor-1 signaling complex." in: Immunity, Vol. 4, Issue 4, pp. 387-96, 1996 (PubMed).