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Details for Product No. ABIN127073

RIP antibody

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Antigen
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Polyclonal
Application
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 2 references available
Catalog no. ABIN127073
Quantity 0.1 mg
Price
357.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic peptide surrounding amino acid 645 of Human RIP
Specificity The antibody detects 74 kDa RIP in Western blot analysis.
Purification Affinity Chromatography.
Background RIP (Receptor Interacting Protein) is a 74 kDa Ser/Thr kinase which interacts with CD95 (Fas/Apo1) receptor and the tumor necrosis factor receptor (TNFR1). It is a cell death domain adapter protein which can bind to the adapter proteins TRADD, RAID, and TRAF2. RIP contains an N-terminal region with homology to protein kinase, an intermediate domain capable of association with MAPKKK and a C-terminal region containing an intracellular death domain motif. RIP activates both p38 MAP kinase and SAPK families. In vitro, RIP induces apoptosis, as well as SAPK/JNK and NF-kB activation. RIP possesses kinase activity as it autophosphorylates itself on serine and threonine residues.
Alternate names: Cell death protein RIP, RIPK1, Receptor-Interacting Protein, Receptor-interacting serine/threonine-protein kinase 1, Serine/threonine-protein kinase RIP
Gene ID 8737
NCBI Accession NP_003795.2
UniProt Q13546
Research Area Cancer, Apoptosis/Necrosis
Application Notes Western Blot: 2-6 µg/ml. Immunoprecipitation. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS, pH 7.2, containing 30% Glycerol, 0.5% BSA as stabilizer and 0.01% Thimerosal as preservative.
Preservative Thimerosal (Merthiolate)
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C/-80 °C
Storage Comment Store the antibody undiluted at -20°C or (in aliquots) at -70°C for long term storage.
Expiry Date 12 months
Background publications Stanger, Leder, Lee et al.: "RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death." in: Cell, Vol. 81, Issue 4, pp. 513-23, 1995 (PubMed).

Hsu, Huang, Shu et al.: "TNF-dependent recruitment of the protein kinase RIP to the TNF receptor-1 signaling complex." in: Immunity, Vol. 4, Issue 4, pp. 387-96, 1996 (PubMed).

Alternatives for antigen "RIP", type "Antibodies"
Hosts (6), (5)
Reactivities (11), (5), (4)
Applications (10), (5), (2), (2), (1)
Epitopes (5), (2), (1)
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