RIPK1 antibody (Middle Region)

Catalog No. ABIN3042930

Product Details anti-RIPK1 Antibody

Target: RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))

Binding Specificity: AA 411-425, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIPK1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Receptor-interacting serine/threonine-protein kinase 1(RIPK1) detection. Tested with WB in Human.

Sequence: RRRRVSHDPF AQQRP

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Receptor-interacting serine/threonine-protein kinase 1(RIPK1) detection. Tested with WB in Human.
Gene Name: receptor(TNFRSF)-interacting serine-threonine kinase 1
Protein Name: Receptor-interacting serine/threonine-protein kinase 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human RIP(411-425aa RRRRVSHDPFAQQRP).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for RIPK1 is approximately 1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-RIPK1 antibody (ABIN3042930)

Wang, Ma, Hu, Xie, Wu, Zeng, Song: "Bifidobacterial recombinant thymidine kinase-ganciclovir gene therapy system induces FasL and TNFR2 mediated antitumor apoptosis in solid tumors." in: BMC cancer, Vol. 16, pp. 545, (2017) (PubMed).

Chen, Zhao, Wu, Zou, Luo, Li, Xie, Liang: "The role of RIP1 and RIP3 in the development of aplastic anemia induced by cyclophosphamide and busulphan in mice." in: International journal of clinical and experimental pathology, Vol. 7, Issue 12, pp. 8411-20, (2015) (PubMed).

Target Details for RIPK1

Target: RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))

Alternative Name: RIPK1 (RIPK1 Products)

Synonyms: RIP antibody, RIP1 antibody, D330015H01Rik antibody, Rinp antibody, Rip1 antibody, rip1 antibody, ripk1 antibody, xRIP1beta antibody, xrip1 antibody, receptor interacting serine/threonine kinase 1 antibody, receptor (TNFRSF)-interacting serine-threonine kinase 1 antibody, receptor interacting serine/threonine kinase 1 L homeolog antibody, RIPK1 antibody, Ripk1 antibody, ripk1.L antibody

Background: RIPK1(Regulator of G Protein Signaling 3), also called RIP, is an enzyme that in humans is encoded by the RIPK1 gene. Members of the TRAF protein family have been implicated in the activation of NF-kappa-B by the TNF superfamily. By yeast 2-hybrid and coimmunoprecipitation studies using mammalian cell extracts, Hsu et al.(1996) showed that RIP interacts with TRADD, TRAF1, TRAF2, and TRAF3. Hartz(2012) mapped the RIPK1 gene to chromosome 6p25.2 based on an alignment of the RIPK1 sequence with the genomic sequence. Stanger et al.(1995) found that overexpression of Rip in mammalian cells induced morphologic changes characteristic of apoptosis. They suggested that RIP may be an important element in the signal transduction machinery that mediates programmed cell death.

Synonyms: Cell death protein RIP antibody|FLJ39204 antibody|OTTHUMP00000039163 antibody|Receptor(TNFRSF) interacting serine threonine kinase 1 antibody|receptor interacting protein 1 antibody|Receptor interacting protein antibody|Receptor interacting serine threonine protein kinase 1 antibody|Receptor TNFRSF interacting serine threonine kinase 1 antibody|Receptor-interacting protein 1 antibody|Receptor-interacting serine/threonine-protein kinase 1 antibody|Rinp antibody|RIP 1 antibody|RIP antibody|Rip-1 antibody|RIP1 antibody|RIPK 1 antibody|RIPK1 antibody|RIPK1_HUMAN antibody|Serine threonine protein kinase RIP antibody|Serine/threonine-protein kinase RIP antibody

UniProt: Q13546

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, Ubiquitin Proteasome Pathway