Insulin Receptor Substrate 1 (IRS1) (AA 298-316), (pSer307) antibody

Details for Product No. ABIN129580
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Antigen
Synonyms irs1, irsu, IRS1, HIRS-1, G972R, IRS-1, IRS1IRM, irs-1, irs1-a
Epitope
AA 298-316, pSer307
(45), (45), (43), (41), (24), (22), (20), (19), (16), (14), (13), (13), (12), (12), (12), (10), (9), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(481), (347), (331), (109), (108), (97), (50), (24), (3), (1)
Host
Rabbit
(493), (15)
Clonality
Polyclonal
Conjugate
Un-conjugated
(11), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10)
Application
Western Blotting (WB), ELISA
(308), (189), (166), (100), (97), (91), (34), (25), (25), (7), (4), (3), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN129580
361.90 $
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Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 298-316 of human IRS1 protein.
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Alternative Name IRS1
Background Insulin Receptor Substrate 1 (IRS1) acts as a signaling molecule for IL-4, insulin and insulin-like growth factor-I (IGF-I) receptors.  When phosphorylated by the insulin receptor, IRS1 binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2.  When bound, IRS1 typically activates phos-phatidylinositol 3-kinase p85 subunit.  IRS1 interacts with both the NPXY motif of tyrosine-phos-phorylated IGF1R and the INSR through the PTB domain.  Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the
Synonyms: HIRS 1 antibody, HIRS1 antibody, Insulin Receptor Substrate 1 antibody, IRS 1 antibody
Gene ID 3667
NCBI Accession NP_005535
UniProt P35568
Research Area Cardiovascular, Atherosclerosis, Signaling, Growth Factors, Cancer
Application Notes This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to phosphorylated IRS1 protein by western blotting in the appropriate cell lysate or extract.   Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pS307 of IRS1 protein.
Restrictions For Research Use only
Format Liquid
Concentration 0.76 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Insulin Receptor Substrate 1 (IRS1) (AA 298-316), (pSer307) antibody Western blot using Affinity Purified anti-IRS1 pS307 antibody shows detection of a band at ~180 kDa believed to represent phosphorylated IRS1 (arrowhead).  Lane 1 shows staining of human 293 cell lysate.  Lane 2 shows staining of 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 µg/ml of anisomysin for 30 min.   The pronounced staining of the band at 180 kDa is not seen when the antibody was pre-incubated with immunizing peptide prior to reaction (data not shown).    The identity of the intensely reactive bands at ~70 kD in both lane 1 and 2 is unknown, although these bands were also competed out by pre-incubation with the immunizing peptide.  Approximately 25 µg of each lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.   After blocking with 5% goat serum, 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:250. Reaction occurred overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature (800 nm channel, green).   Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).   IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.
General Shah, Hunter: "Turnover of the active fraction of IRS1 involves raptor-mTOR- and S6K1-dependent serine phosphorylation in cell culture models of tuberous sclerosis." in: Molecular and cellular biology, Vol. 26, Issue 17, pp. 6425-34, 2006 (PubMed).

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