GFP antibody (Green Fluorescent Protein) (AA 246) (HRP)

Details for Product anti-GFP Antibody No. ABIN129721, Supplier: Log in to see
Antigen
  • green fluorescent protein
  • gfp
Epitope
AA 246
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Reactivity
Aequorea victoria
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This GFP antibody is conjugated to HRP
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Application
Fluorescence Microscopy (FM), ELISA, Western Blotting (WB)
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Immunogen The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.
Immunogentype:Recombinant
Isotype IgG
Cross-Reactivity recombinant GFP (rGFP), enhanced GFP (eGFP)
Characteristics Concentration Definition: by UV absorbance at 280 nm
Alternative Name GFP
Background Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Synonyms: HRP, GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.
Research Area Tags/Labels
Application Notes Anti-GFP Antibody is designed to detect GFP and its variants. This GFP antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Gentamicin sulfate
Handling Advice Do NOT add Sodium Azide!
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) (AA 246) antibody (HRP) (ABIN129721) Western Blot of Anti-GFP Antibody Peroxidase Conjugate. Lane 1: 50ng of GFP. Lane 2: ...
Product cited in: Maloverjan, Piirsoo, Kasak, Peil, Østerlund, Kogerman: "Dual function of UNC-51-like kinase 3 (Ulk3) in the Sonic hedgehog signaling pathway." in: The Journal of biological chemistry, Vol. 285, Issue 39, pp. 30079-90, 2010 (PubMed).

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