PRAME Protein (AA 1-509) (GST tag)
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- Target See all PRAME Proteins
- PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-509
- Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This PRAME protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- PRAME (Human) Recombinant Protein (P01)
- Sequence
- MERRRLRGSI QSRYISMSVW TSPRRLVELA GQSLLKDEAL AIAALELLPR ELFPPLFMAA FDGRHSQTLK AMVQAWPFTC LPLGVLMKGQ HLHLETFKAV LDGLDVLLAQ EVRPRRWKLQ VLDLRKNSHQ DFWTVWSGNR ASLYSFPEPE AAQPMTKKRK VDGLSTEAEQ PFIPVEVLVD LFLKEGACDE LFSYLIEKVK RKKNVLRLCC KKLKIFAMPM QDIKMILKMV QLDSIEDLEV TCTWKLPTLA KFSPYLGQMI NLRRLLLSHI HASSYISPEK EEQYIAQFTS QFLSLQCLQA LYVDSLFFLR GRLDQLLRHV MNPLETLSIT NCRLSEGDVM HLSQSPSVSQ LSVLSLSGVM LTDVSPEPLQ ALLERASATL QDLVFDECGI TDDQLLALLP SLSHCSQLTT LSFYGNSISI SALQSLLQHL IGLSNLTHVL YPVPLESYED IHGTLHLERL AYLHARLREL LCELGRPSMV WLSANPCPHC GDRTFYDPEP ILCPCFMPN
- Characteristics
- Human PRAME full-length ORF ( AAH39731, 1 a.a. - 509 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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Functional autoantibodies against SSX-2 and NY-ESO-1 in multiple myeloma patients after allogeneic stem cell transplantation." in: Cancer immunology, immunotherapy : CII, Vol. 63, Issue 11, pp. 1151-62, (2014) (PubMed).
: "
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Functional autoantibodies against SSX-2 and NY-ESO-1 in multiple myeloma patients after allogeneic stem cell transplantation." in: Cancer immunology, immunotherapy : CII, Vol. 63, Issue 11, pp. 1151-62, (2014) (PubMed).
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- Target
- PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
- Alternative Name
- PRAME (PRAME Products)
- Synonyms
- PRAME Protein, CT130 Protein, MAPE Protein, OIP-4 Protein, OIP4 Protein, 4930534P07Rik Protein, preferentially expressed antigen in melanoma Protein, PRAME Protein, Prame Protein
- Background
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Full Gene Name: preferentially expressed antigen in melanoma
Synonyms: MAPE,OIP4 - Gene ID
- 23532
- Pathways
- Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding
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