CD11b antibody (Integrin alpha M)

Details for Product anti-ITGAM Antibody No. ABIN135141, Supplier: Log in to see
Antigen
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha M
  • integrin, alpha M
  • ITGAM
  • Itgam
Alternatives
anti-Mouse (Murine) CD11b antibody for Biochemical Assay
Reactivity
Mouse (Murine)
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473
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2
Host
Rat
495
370
164
16
5
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD11b antibody is un-conjugated
147
119
88
48
20
19
15
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1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
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139
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67
25
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13
8
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1
1
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1
1
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1
Options
Supplier
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Immunogen C57BL/10 mouse splenic T cells and concanavaline A-activated C57BL/10 splenocytes1
Clone M1-70
Isotype IgG2b
Characteristics The antibody detects: M subunit (Mr 170 kDa) of the CD11b/CD18 heterodimer
Cross-reaction of M1/70 to human CD11b has been observed on monocytes, polymorphonuclear leukocytes, and NK cells.
Endotoxin Level Low Endotoxin
Alternative Name CD11b/Mac-1a (ITGAM Antibody Abstract)
Background CD11b, the integrin M subunit, combines with CD18, the integrin 2 subunit, to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1/CD54. Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 expression is rapidly up-regulated on neutrophils after activation and in the same time period that CD62L is shed from the cell surface. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils.
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
Application Notes Working Dilution:
FACS: 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

In vitro and in vivo blocking

Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS.
Preservative Azide free
Handling Advice Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage -20 °C
Storage Comment Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.
Background publications Gyetko, Sud, Kendall, Fuller, Newstead, Standiford: "Urokinase receptor-deficient mice have impaired neutrophil recruitment in response to pulmonary Pseudomonas aeruginosa infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 3, pp. 1513-9, 2000 (PubMed).

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, 1994 (PubMed).

Flotte, Springer, Thorbecke: "Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets." in: The American journal of pathology, Vol. 111, Issue 1, pp. 112-24, 1983 (PubMed).

Springer, Galfrè, Secher, Milstein: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978 (PubMed).