Lysosomal-Associated Membrane Protein 1 (LAMP1) antibody (FITC)

Details for Product No. ABIN135443
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Antigen
Synonyms AI196048, CD107a, Lamp-1, LAMPA, LGP120, LEP100
Reactivity
Mouse (Murine)
(133), (69), (59), (7), (7), (3), (3), (2)
Host
Rat
(92), (74), (30), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
(21), (15), (15), (10), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC)
(111), (86), (44), (39), (37), (33), (29), (22), (19), (10), (1), (1)
Pubmed 2 references available
Quantity 0.5 mg
Options
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Catalog No. ABIN135443
335.50 $
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Immunogen Plasma membrane fraction of mouse embryo NIH3T3 cell line1
Clone 1D4B
Isotype IgG2a
Characteristics The antibody detects: Murine CD107a/LAMP-1 (Mr 110-140 kDa with a core protein of Mr approx. 40 kDa)
Alternative Name CD107a/LAMP-1
Background CD107a, also known as lysosomal-associated membrane protein 1 (LAMP-1), is a heavily glycosylated, type I transmembrane protein that constitutes one of the two major sialoglycoproteins on lysosomal membranes that can be used to distinguish lysosomes from other organelles. It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion. CD107a is principally expressed in epithelial cells and macrophages in a variety of organs in normal and Beige mutant mice. LAMP-1 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases.
Application Notes Working Dilution:
FACS: 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

Immunoelectron microscopy, Immunoaffinity chromatography

Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.5 mg in 1.0 mL of PBS / NaN3.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
General Chen, Pan, DSouza et al.: "Lysosome-associated membrane proteins: characterization of LAMP-1 of macrophage P388 and mouse embryo 3T3 cultured cells." in: Archives of biochemistry and biophysics, Vol. 239, Issue 2, pp. 574-86, 1985 (PubMed).

Rohrer, Schweizer, Russell et al.: "The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane." in: The Journal of cell biology, Vol. 132, Issue 4, pp. 565-76, 1996 (PubMed).

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