CD4 Molecule (CD4) antibody (PE)

Antigen: CD4 Molecule (CD4)

Synonyms: CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25

Clonality: Monoclonal (CT-4)

Host: Mouse

Reactivity: Chicken, Bird (Avian)

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN135511

Additional Information

Alternative name: CD4

Isotype: IgG1

Clone: CT-4

Description: Chicken CD4 (Mr 64 kDa) CD4 is a type I transmembrane glycoprotein expressed on approximately 70% of thymocytes, 10% of spleen cells and 45% of peripheral blood lymphocytes, but <1% of bursal cells.1 The monoclonal antibody CT-4 inhibits both PHA- and Con A-induced proliferative responses of splenocytes, and PWM-induced IL-2 production.1 This antibody also reacts with turkey CD4 as demonstrated by flow cytometry and immunoprecipitation/western blotting.

Application Details

Application Notes: WORKING DILUTIONS Flow Cytometry:Fluorescein (1 ug/10^6 cells), Biotin (1 ug/10^6 cells), R-phycoerythrin (0.2 ug/10^6 cells). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

anti-CD4 Molecule (CD4) antibody (PE)

Publications

Publications: Chan, Chen, Ager et al.: "Identification of the avian homologues of mammalian CD4 and CD8 antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 7, pp. 2133-8, 1988 (PubMed).

Dalgaard, Norup, Pedersen et al.: "Flow cytometric assessment of chicken T cell-mediated immune responses after Newcastle disease virus vaccination and challenge." in: Vaccine, 2010 (PubMed).

Parvizi, Andrzejewski, Read et al.: "Expression profiling of genes associated with regulatory functions of T-cell subsets in Marek's disease virus-infected chickens." in: Avian pathology : journal of the W.V.P.A, Vol. 39, Issue 5, pp. 367-73, 2010 (PubMed).

Carballeda, Zoth, Gómez et al.: "Activation of the immune response against Infectious Bursal Disease Virus after intramuscular inoculation of an intermediate strain." in: Immunobiology, Vol. 216, Issue 9, pp. 1028-33, 2011 (PubMed).