RUNX1 antibody (pSer249) (Cy5)
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- Target See all RUNX1 Antibodies
- RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))
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Binding Specificity
- pSer249
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RUNX1 antibody is conjugated to Cy5
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Application
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- Cross-Reactivity
- Mouse
- Predicted Reactivity
- Human,Rat,Dog,Cow,Pig,Rabbit,Guinea Pig
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic phosphopeptide derived from human RUNX1 around the phosphorylation site of Ser249 [QP(p-S)PP]
- Isotype
- IgG
- Top Product
- Discover our top product RUNX1 Primary Antibody
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- Application Notes
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IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))
- Alternative Name
- RUNX1 (RUNX1 Products)
- Synonyms
- RUNX1 antibody, runx1 antibody, AI462102 antibody, AML1 antibody, Cbfa2 antibody, Pebp2a2 antibody, Pebpa2b antibody, AML1-EVI-1 antibody, AMLCR1 antibody, CBFA2 antibody, EVI-1 antibody, PEBP2aB antibody, runxa antibody, Runx-1 antibody, XAML antibody, Xaml1 antibody, aml antibody, aml-1 antibody, aml1 antibody, aml1-evi-1 antibody, amlcr1 antibody, cbfa2 antibody, evi-1 antibody, pebp2ab antibody, Aml1 antibody, uncharacterized LOC473981 antibody, runt-related transcription factor antibody, runt related transcription factor 1 antibody, runt-related transcription factor 1 antibody, runt related transcription factor 1 L homeolog antibody, LOC473981 antibody, runt antibody, Runx1 antibody, RUNX1 antibody, runx1 antibody, runx1.L antibody
- Background
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Synonyms: RUNX1 Ser249, p-RUNX1 Ser249, RUNX1 phospho S249, RUNX1 phospho Ser249, Acute myeloid leukemia 1, Acute myeloid leukemia 1 protein, alpha subunit core binding factor, AML 1, AML1 EVI 1, AML1, Aml1 oncogene, AMLCR 1, AMLCR1, CBFA 2, CBFA2, Core binding factor alpha 2 subunit, Core binding factor runt domain alpha subunit 2, EVI 1, EVI1, HGNC, Oncogene AML 1, PEA2 alpha, PEBP2 alpha B, PEBP2A2, PEBP2aB, Polyomavirus enhancer binding protein 2 alpha B subunit, Run1, Runt related transcription factor 1, RUNX 1, SL3 3 enhancer factor 1 alpha B subunit, SL3/AKV core binding factor alpha B subunit, RUNX1_HUMAN.
Background: AML1/Runx1 binds DNA as a monomer and through the Runt domain. DNA binding is increased by heterodimerization with CBFB. Isoform AML1L can neither bind DNA nor heterodimerize and interferes with the transactivation activity of AML1/Runx1. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T cell receptor enhancers, LCK, IL3 and GMCSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. AML1/Runx1 is expressed in a wide variety of tissues and is expressed at the highest levels in thymus, bone marrow and peripheral blood. Defects in AML1/Runx1 are the cause of familial platelet disorder with associated myeloid malignancy, an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.
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