Cytochrome B-245, alpha Polypeptide (CYBA) antibody (FITC)

Details for Product No. ABIN1449194, Supplier: Log in to see
Antigen
  • zgc:66077
  • MGC80750
  • CYBA
  • p22-phox
  • p22phox
  • p22-PHOX
  • b558
  • nmf333
  • P22-PHOX
  • cytochrome b-245, alpha polypeptide
  • cytochrome b-245 light chain-like
  • p22-phox
  • cyba
  • CYBA
  • LOC101328418
  • Cyba
Alternatives
anti-Human Cytochrome B-245, alpha Polypeptide antibody for Western Blotting
Reactivity
Human
49
23
23
4
2
2
2
1
1
1
Host
Mouse
45
4
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
32
18
16
13
5
4
3
2
1
1
1
Options
Supplier
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Immunogen Human cytochrome b rich fraction
Clone 7D5
Isotype IgG1
Specificity This antibody reacts with an extracellular epitope of gp91phox. The antibody recognizes the epitope in the native conformation. This antibody reacts with Flavocytochrome b558 on flow cytometry.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Protein A agarose
Alternative Name Cytochrome B-245 Light Chain (CYBA Antibody Abstract)
Background The NADPH oxidase is a multicomponent enzyme that transfers electrons from NADPH to O2 to generate superoxide (O2 -), a key part of the phagocytic or neutrophilic respiratory burst response. Flavocytochrome b558 is the catalytic component of the phagocyte NADPH oxidase. It is a transmembrane heterodimer composed of a large glycoprotein, gp91phox (PHagocyte OXidase) and a smaller protein, p22phox. Upon cell stimulation, flavocytochrome b558 assembles with p67phox, p47phox, and the GTP-binding protein Rac and becomes activated to generate O2 -. Mutations in gp91phox, p22phox, or other components of the NADPH oxidase can result in chronic granulomatous disease, which is associated with significant morbidity and mortality due to a predisposition to recurrent bacterial and fungal infections.Synonyms: CYBA, Cytochrome b(558) alpha chain, Cytochrome b558 subunit alpha, Neutrophil cytochrome b 22 kDa polypeptide, Superoxide-generating NADPH oxidase light chain subunit, p22 phagocyte B-cytochrome, p22-phox
Gene ID 1535
NCBI Accession NP_000092
UniProt P13498
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones, Carbohydrate Homeostasis
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS, 0.09 % NaN3, 1 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 2-8 °C.
Supplier Images
Flow Cytometry (FACS) image for anti-Cytochrome B-245, alpha Polypeptide (CYBA) antibody (FITC) (ABIN1449194) anti-Cytochrome B-245, alpha Polypeptide (CYBA) antibody (FITC)
Background publications Kornberg: "Remembering our teachers." in: The Journal of biological chemistry, Vol. 276, Issue 1, pp. 3-11, 2001 (PubMed).

Yamauchi, Yu, Pötgens, Kuribayashi, Nunoi, Kanegasaki, Roos, Malech, Dinauer, Nakamura: "Location of the epitope for 7D5, a monoclonal antibody raised against human flavocytochrome b558, to the extracellular peptide portion of primate gp91phox." in: Microbiology and immunology, Vol. 45, Issue 3, pp. 249-57, 2001 (PubMed).

Burritt, DeLeo, McDonald, Prigge, Dinauer, Nakamura, Nauseef, Jesaitis: "Phage display epitope mapping of human neutrophil flavocytochrome b558. Identification of two juxtaposed extracellular domains." in: The Journal of biological chemistry, Vol. 276, Issue 3, pp. 2053-61, 2001 (PubMed).

Yu, Cross, Zhen, Dinauer: "Functional analysis of NADPH oxidase in granulocytic cells expressing a delta488-497 gp91(phox) deletion mutant." in: Blood, Vol. 94, Issue 7, pp. 2497-504, 1999 (PubMed).

Yu, Quinn, Cross, Dinauer: "Gp91(phox) is the heme binding subunit of the superoxide-generating NADPH oxidase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 14, pp. 7993-8, 1998 (PubMed).

Strader, Gaffney, Sugg, Candelore, Keys, Patchett, Dixon: "Allele-specific activation of genetically engineered receptors." in: The Journal of biological chemistry, Vol. 266, Issue 1, pp. 5-8, 1991 (PubMed).

Verhoeven, Bolscher, Meerhof, van Zwieten, Keijer, Weening, Roos: "Characterization of two monoclonal antibodies against cytochrome b558 of human neutrophils." in: Blood, Vol. 73, Issue 6, pp. 1686-94, 1989 (PubMed).

Nakamura, Murakami, Koga, Tanaka, Minakami: "Monoclonal antibody 7D5 raised to cytochrome b558 of human neutrophils: immunocytochemical detection of the antigen in peripheral phagocytes of normal subjects, patients with chronic granulomatous disease, and their carrier mothers." in: Blood, Vol. 69, Issue 5, pp. 1404-8, 1987 (PubMed).

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