CHEK1 antibody (Ser280)

Catalog No. ABIN1574028

Product Details anti-CHEK1 Antibody

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Binding Specificity: Ser280

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Rabbit Anti-Chk1 (Ser280) Polyclonal Antibody detects endogenous levels of human Chk1 protein.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide (KLH-coupled) derived from AA surrounding Ser280 of human Chk1

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL Western blot: 0.5-2 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: Chk1 (CHEK1 Products)

Synonyms: An08g10320 antibody, AO090003000441 antibody, CHK1 antibody, chk1 antibody, C85740 antibody, Chk1 antibody, rad27 antibody, id:ibd2720 antibody, zgc:56093 antibody, CHEK1 antibody, checkpoint kinase 1 antibody, serine/threonine-protein kinase chk1 antibody, serine/threonine-protein kinase Chk1 antibody, CAMK/CAMKL/CHK1 protein kinase Chk1 antibody, checkpoint kinase 1 S homeolog antibody, CHEK1 antibody, ANI_1_2436074 antibody, AOR_1_768154 antibody, PTRG_04183 antibody, SJAG_01680 antibody, PAAG_04978 antibody, MCYG_03290 antibody, VDBG_03742 antibody, chek1.S antibody, Chek1 antibody, chek1 antibody

Background: Chk1 is a serine/threonine kinase first identified as a checkpoint kinase in Schizosaccharomyces pombe. Chk1 is an evolutionarily conserved protein kinase that functions to ensure genomic integrity upon genotoxic stress. Chk1 is activated through phosphorylation by ATR/ATM and plays an important role in DNA damage checkpoint control, embryonic development, and tumor suppression. The activation of Chk1 in response to DNA replication blockages and certain forms of genotoxic stress involves phosphorylation of Ser317 and Ser345. Chk1 is also phosphorylated at Ser280 and Ser296 following DNA damage. Activated Chk1 further activates downstream factors that mediate cell cycle arrest and other cell physiological events through phosphorylation. These key cell cycle factors include p53, Cdc25C and more. Rabbit Anti-Chk1 (Ser280) Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide derived from the residues surrounding Ser 280 of human Chk1 (Swiss Prot: O14757).

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair