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Amylase, alpha antibody (Biotin)

AMY Reactivity: Bacillus amyloliquefaciens WB, ELISA, IP Host: Rabbit Polyclonal Biotin
Catalog No. ABIN1607777
  • Target See all Amylase, alpha (AMY) Antibodies
    Amylase, alpha (AMY)
    Reactivity
    • 9
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Bacillus amyloliquefaciens
    Host
    • 16
    • 2
    Rabbit
    Clonality
    • 17
    • 1
    Polyclonal
    Conjugate
    • 9
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    This Amylase, alpha antibody is conjugated to Biotin
    Application
    • 11
    • 9
    • 8
    • 6
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Cross-Reactivity (Details)
    Cross reactivity against a-Amylase from other sources may occur but has not been specifically determined.
    Purity
    Anti-Alpha Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified a-Amylase [B.amyloliquefaciens].
    Endotoxin Level
    Low Endotoxin : No
    Immunogen
    a-Amylase [Bacillus amyloliquefaciens]
    Immunogen Type: Native Protein
    Isotype
    IgG
  • Application Notes
    Anti-Alpha Amylase has been assayed against 1.0 µg of a-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:20.000
    IF Immunoprecipitation Dilution: 1:100
    Western Blot Dilution: 1:500 - 1:5.000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    10 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Handling Advice
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Expiry Date
    Expiration date is one (1) year from date of opening.
  • Target
    Amylase, alpha (AMY)
    Alternative Name
    Alpha Amylase (AMY Products)
    Synonyms
    LOC663954 antibody, LOC100136478 antibody, alpha amylase antibody, alpha-amylase antibody, LOC663954 antibody, HQ_RS05305 antibody, LOC100136478 antibody
    Background
    Alpha Amylase is a member of the glycosyl hydrolase 13 family, involved primarily in the hydrolysis of biopolymer residues of 1, 4-alpha-D-glucosidic linkages in polysaccharides. It performs its catalytic function in metabolism of carbohydrates. Anti-Alpha Amylase antibody is ideal for investigators involved in Metabolism, Signal Transduction, and Cancer research. Synonyms: Alpha-amylase EC=3.2.1.1 1,4-alpha-D-glucan glucanohydrolase
    Gene ID
    7849308
    UniProt
    P00692
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