SNCA antibody (AA 1-140)

Catalog No. ABIN1715843

Product Details anti-SNCA Antibody

Target: SNCA (Synuclein, alpha (SNCA))

Binding Specificity: AA 1-140

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNCA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purpose: Polyclonal Antibody to Synuclein Alpha (SNCa)

Sequence: MGHHHHHHSG SEFELRRQAC - MKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Specificity: The antibody is a rabbit polyclonal antibody raised against SNCa. It has been selected for its ability to recognize SNCa in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Synuclein Alpha (SNCa) corresdonding to Met1~Ala140 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-SNCA antibody (ABIN1715843)

Bhattamisra, Shak, Xi, Safian, Choudhury, Lim, Shahzad, Alhakamy, Anwer, Radhakrishnan, Md: "Nose to brain delivery of rotigotine loaded chitosan nanoparticles in human SH-SY5Y neuroblastoma cells and animal model of Parkinson's disease." in: International journal of pharmaceutics, Vol. 579, pp. 119148, (2020) (PubMed).

Target Details for SNCA

Target: SNCA (Synuclein, alpha (SNCA))

Alternative Name: Synuclein Alpha (SNCA Products)

Synonyms: snca antibody, MGC64356 antibody, LOC619283 antibody, NACP antibody, alphaSYN antibody, PARK1 antibody, PARK4 antibody, PD1 antibody, synuclein alpha L homeolog antibody, alpha-synuclein antibody, synuclein alpha antibody, synuclein, alpha antibody, snca.L antibody, LOC619283 antibody, SNCA antibody, Snca antibody

Background: SNC-A, aSYN, PD1, PARK1, PARK4, NACP, Non-A Beta Component Of Alzheimer's Disease Amyloid Precursor Protein, Non-A4 component of amyloid precursor

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity