MAP2 antibody (AA 2-309)

Catalog No. ABIN1742388

Product Details anti-MAP2 Antibody

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Binding Specificity: AA 2-309

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Specific for MAP 2, recognizes all three isoforms.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant mouse MAP 2 (aa 2-309).

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000
IHC: 1 : 250 up to 1 : 500
IHC-P: 1 : 250 up to 1 : 500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-MAP2 antibody (ABIN1742388)

Michalski, Härtig, Krügel, Edwards, Böddener, Böhme, Pannicke, Reichenbach, Grosche: "Region-specific expression of vesicular glutamate and GABA transporters under various ischaemic conditions in mouse forebrain and retina." in: Neuroscience, Vol. 231, pp. 328-44, (2013) (PubMed).

Ribic, Flügge, Schlumbohm, Mätz-Rensing, Walter, Fuchs: "Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey." in: Behavioral and brain functions : BBF, Vol. 7, pp. 1, (2011) (PubMed).

Troca-Marín, Alves-Sampaio, Montesinos: "An increase in basal BDNF provokes hyperactivation of the Akt-mammalian target of rapamycin pathway and deregulation of local dendritic translation in a mouse model of Down's syndrome." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 26, pp. 9445-55, (2011) (PubMed).

Bartoi, Rigbolt, Du, Köhr, Blagoev, Kornau: "GABAB receptor constituents revealed by tandem affinity purification from transgenic mice." in: The Journal of biological chemistry, Vol. 285, Issue 27, pp. 20625-33, (2010) (PubMed).

Target Details for MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Alternative Name: MAP 2 (MAP2 Products)

Synonyms: MAP2A antibody, MAP2B antibody, MAP2C antibody, MAP2R antibody, Mtap2 antibody, MAP-2 antibody, Mtap-2 antibody, xmap2 antibody, MGC53131 antibody, a730034c02 antibody, zgc:103474 antibody, zgc:113974 antibody, microtubule associated protein 2 antibody, microtubule-associated protein 2 antibody, microtubule associated protein 2 S homeolog antibody, MAP2 antibody, Map2 antibody, map2.S antibody, map2 antibody