ITGA4 antibody

Catalog No. ABIN181259

Product Details anti-ITGA4 Antibody

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITGA4 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects Integrin, Alpha 4 (antigen CD49D, Alpha 4 Subunit Of VLA-4 Receptor) (ITGA4) at internal domain.

Purification: Immunoaffinity Chromatography.

Immunogen: ITGA4 antibody was raised against synthetic peptide from internal domain of human ITGA4 / VLA-4. Genename: ITGA4

Application Details

Application Notes: Immunohistochemistry on Paraffin Embedded Sections: 10 μg/mL. Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer.

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: CD49d / ITGA4 (ITGA4 Products)

Synonyms: CD49D antibody, IA4 antibody, LOC100219853 antibody, integrin subunit alpha 4 antibody, integrin alpha 4 antibody, integrin subunit alpha 4 S homeolog antibody, ITGA4 antibody, Itga4 antibody, itga4.S antibody

Background: ITGA4 encodes integrin alpha chain 4. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Although alpha 4 is variably cleaved to yield two unlinked chains, this cleavage is unnecessary for integrin assembly and function. Alpha 4 combines with beta 1 (ITGB1) on T-cells to form the integrin very late (activation) antigen 4 (VLA-4) that can bind to the extracellular matrix molecules fibronectin or thrombospondin, and is also a ligand for the cell surface molecule vascular cell adhesion molecule 1 (VCAM-1). In addition, alpha 4 combines with beta 7 to form the lymphocyte homing receptor known as LPAM-1 (lymphocyte Peyer Patch adhesion molecule 1). Integrins are also known to participate in cell-surface mediated signalling.Synonyms: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4

Gene ID: 3676

NCBI Accession: NP_000876

UniProt: P13612

Pathways: Integrin Complex