MAP2K3 antibody (pSer189)

Catalog No. ABIN1842396

Product Details anti-MAP2K3 Antibody

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Binding Specificity: pSer189

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2K3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity: Rabbit Anti-MKK3 (Phospho-Ser189) Polyclonal Antibody detects endogenous levels of MKK3 only when phosphorylated at serine189.

Characteristics: Molecular Weight: Predicted Band Size 39 kDa, Observed Band Size 39 kDa

Purification: The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Synthesized phosphopeptide derived from human MKK3 around pSer189 (V-D-SP-V-A)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:1,000,
IHC: 1:50-1:100,
IF: 1:100-1:200

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20 °C for 1 year.

Target Details for MAP2K3

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Alternative Name: MKK3 (MAP2K3 Products)

Synonyms: MAPKK3 antibody, MEK3 antibody, MKK3 antibody, PRKMK3 antibody, SAPKK-2 antibody, SAPKK2 antibody, AW212142 antibody, Prkmk3 antibody, mMKK3b antibody, Mkk3 antibody, mitogen-activated protein kinase kinase 3 antibody, mitogen activated protein kinase kinase 3 antibody, MAP kinase activator XMEK3 antibody, MAP2K3 antibody, Map2k3 antibody, map2k3 antibody

Pathways: MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2