PKC delta antibody (pSer645)

Catalog No. ABIN1842452

Product Details anti-PKC delta Antibody

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Binding Specificity: pSer645

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC delta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Rabbit Anti PKCdelta, (Phospho-Ser645) detects endogenous levels of PKCdelta, only when phosphorylated at serine 645.

Characteristics: Molecular Weight: Predicted Band Size 78 kDa, Observed Band Size 78 kDa

Purification: The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Synthesized phosphopeptide derived from human PKCdelta, around pSer645 (R-L-SP-Y-S)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:1,000,
IHC: 1:50-1:100

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20 °C for 1 year.

Target Details for PKC delta

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Alternative Name: PKCdelta (PKCd Products)

Synonyms: MAY1 antibody, PKCD antibody, nPKC-delta antibody, AI385711 antibody, D14Ertd420e antibody, PKC[d] antibody, PKCdelta antibody, Pkcd antibody, fb09d10 antibody, fv43b11 antibody, prkcd antibody, wu:fb09d10 antibody, wu:fv43b11 antibody, zgc:56558 antibody, PKC-delta antibody, may1 antibody, npkc-delta antibody, pkc-delta2 antibody, pkcd antibody, tpa-1 antibody, protein kinase C delta antibody, protein kinase C, delta antibody, protein kinase C, delta a antibody, protein kinase C delta L homeolog antibody, PRKCD antibody, Prkcd antibody, prkcda antibody, prkcd.L antibody, prkcd antibody

Pathways: Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, Lipid Metabolism