Signal Transducer and Activator of Transcription 3 (STAT3) antibody

Antigen: Signal Transducer and Activator of Transcription 3 (STAT3)

Synonyms: APRF, HIES, FLJ20882, MGC16063, Aprf, AW109958, 1110034C02Rik, MGC93551, MGC128731, STAT3, stat3

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184593

Additional Information

Alternative name: STAT3 (isoform 1 and 2)

Gene ID: 6774, 20848 (mouse), 25125 (rat)

Immunogen: DMELTSECATSPM

Format: Affinity-purified

Description: All names and symbols: STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, DNA-binding protein APRF, acute-phase response factor, FLJ20882, MGC16063. Official Symbol: STAT3. RefSeq number(s): NP_003141.2, NP_644805.1. Gene ontology terms: hematopoietin/interferon-class (D200-domain) cytokine receptor signal transducer activity, transcription factor activity, JAK-STAT cascade, acute-phase response, neurogenesis, cell motility, signal transduction, regulation of transcription from Pol II promoter, negative regulation of transcription from Pol II promoter, cytoplasm, nucleus

Specificity: This antibody is expected to recognise isoforms 1 and 2 (as represented by NP_644805.1 and NP_003141.2 respectively) Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx. 90kDa band observed in lysates of NIH3T3 (calculated MW of 88.0kDa according to NP_644805.1 and of 88.1 according to NP_003141.2). Recommended concentration: 1-3µg/ml. Immunohistochemistry: Stains mouse thymic medulla and not the cortex. Recommended overnight staining at 10ug/ml, see picture.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184593 (1µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN184593 overnight staining at 10ug/ml on 4%PFA-fixed mouse thymus. Biotinylated mouse anti-goat then Streptavidin-AP then BCIP/NBT. Data kindly provided by Dr. Marella de Bruijn, MRC Mol. Haematol. Unit, WIMM, JR Hospital, Oxford.

Publications

Publications: Bromberg, Wrzeszczynska, Devgan et al.: "Stat3 as an oncogene." in: Cell, Vol. 98, Issue 3, pp. 295-303, 1999 (PubMed).