PYD and CARD Domain Containing (PYCARD) (C-Term) antibody

Details for Product No. ABIN184674
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Antigen
Synonyms ASC, CARD5, TMS, TMS-1, TMS1, 9130417A21Rik, Asc, TNS1, masc, asc1, zAsc, zgc:109874
Epitope
C-Term
(14), (5), (5), (5), (3), (3), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(62), (21), (18), (12), (12), (12), (8), (1)
Host
Goat
(49), (12), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(57), (17), (17), (12), (10), (10), (5), (5), (4), (3), (3), (3), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN184674
374.27 $
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Immunogen Peptide with sequence C-RESQSYLVEDLERS, from the C Terminus of the protein sequence according to NP_037390.2, NP_660183.1.
Specificity This antibody is expected to recognise all reported Human isoforms according to NP_037390.2 and NP_660183.1.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ASC / TMS1
Background Synonyms: PYCARD, ASC, TMS1, PYD and CARD domain containing, CARD5, MGC10332, TMS, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain protein 5, target of methylation-induced silencing-1, TMS-1
Gene ID 29108
NCBI Accession NP_660183, NP_037390
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 22 kDa band observed in Hela and Human Kidney lysates (predicted MW of 22 kDa according to NP_037390.2). Recommended for use at 1-3 µg/mL.
IHC: In paraffin embedded Human Liver shows staining of sinusoidal lining cells. Recommended concentration: 2-4 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-PYD and CARD Domain Containing (PYCARD) (C-Term) antibody ABIN184674 (1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Ohtsuka, Ryu, Minamishima et al.: "ASC is a Bax adaptor and regulates the p53-Bax mitochondrial apoptosis pathway." in: Nature cell biology, Vol. 6, Issue 2, pp. 121-8, 2004 (PubMed).

Validation Images
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