You are viewing an incomplete version of our website. Please click to reload the website as full version.

Numb Homolog (NUMB) (C-Term) antibody

Details for Product No. ABIN184679, Supplier: Log in to see
Antigen
  • Nb
  • C14orf41
  • S171
  • c14_5527
Epitope
C-Term
32
31
13
13
9
8
6
6
6
5
5
3
3
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
135
60
25
6
5
5
2
2
2
2
1
1
Host
Goat
84
50
15
3
Clonality
Polyclonal
Conjugate
Un-conjugated
5
4
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
125
38
23
21
18
12
10
10
8
7
4
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Immunogen Peptide with sequence C-PFSSDLQKTFEIEL, from the C Terminus of the protein sequence according to NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1.
Blocking Peptide Blocking peptide for this product available: ABIN369541
Specificity This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.
Cross-Reactivity Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name NUMB (NUMB Antibody Abstract)
Background Synonyms: NUMB, numb homolog (Drosophila), S171, numb homolog
Gene ID 8650, 18222
NCBI Accession NP_001005743, NP_003735, NP_001005745
Research Area Stem Cells
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 80 kDa band observed in Mouse Brain lysates (calculated MW of 70.8 kDa according to NP_001005743.1). Recommended concentration: 0.03-0.1 µg/mL. Immunocytochemsitry/Immunofluorescence: This antibody has been successfully used in mouse hematopoetic stem cells,. in Mouse Embryonic brain tissue and in Human keratinocytes. Immunoprecipitation: An anonymous customer found positive results in IP on Mouse Embryo.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Numb Homolog (NUMB) (C-Term) antibody (ABIN184679) ABIN184679 staining (0.03µg/ml) of Mouse Brain lysate (RIPA buffer, 30µg total protei...
Background publications Holowacz, Zeng, Lassar: "Asymmetric localization of numb in the chick somite and the influence of myogenic signals." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 235, Issue 3, pp. 633-45, 2006 (PubMed).

Sanada, Tsai: "G protein betagamma subunits and AGS3 control spindle orientation and asymmetric cell fate of cerebral cortical progenitors." in: Cell, Vol. 122, Issue 1, pp. 119-31, 2005 (PubMed).

Zhong, Feder, Jiang et al.: "Asymmetric localization of a mammalian numb homolog during mouse cortical neurogenesis." in: Neuron, Vol. 17, Issue 1, pp. 43-53, 1996 (PubMed).