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FOXA1 antibody (Forkhead Box A1) (C-Term)

Details for Product anti-FOXA1 Antibody No. ABIN185021, Supplier: Log in to see
Antigen
  • FoxA4a
  • XFD-1
  • fkh1
  • foxa1
  • pintallavis
  • xfkh1
  • xkfh-1
  • HNF3-alpha
  • HNF3alpha
  • Hnf3a
  • fkh2
  • tFoxA1
  • tcf3a
  • xfkh2
  • fkh
  • FOXA1
  • Ci-fkh
  • foxa3
  • Foxa1
  • fkd7
  • forkhead-7
  • hnf3a
  • HNF3A
  • TCF3A
  • HNF-3a
  • Hnf-3a
  • Tcf-3a
  • Tcf3a
Epitope
C-Term
29
19
16
16
13
13
9
7
6
5
4
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
146
33
27
7
6
5
3
2
1
1
1
Host
Goat
70
56
20
Clonality
Polyclonal
Conjugate
This FOXA1 antibody is un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
116
77
44
35
25
11
9
9
6
5
3
2
1
1
1
Supplier
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Immunogen Peptide with sequence C-GVYSRPVLNTS, from the C Terminus of the protein sequence according to NP_004487.2.
Blocking Peptide Blocking peptide for this product available: ABIN369148
Cross-Reactivity Rat (Rattus), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Alternative Name FOXA1 / HNF3A (FOXA1 Antibody Abstract)
Background Synonyms: FOXA1, HNF3A, forkhead box A1, TCF3A, MGC33105, hepatocyte nuclear factor 3, alpha
Gene ID 3169, 15375, 25098
NCBI Accession NP_004487
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 50 kDa band observed in Human lysates of cell line HepG2 (calculated MW of 49.1 kDa according to NP_004487.2). Recommended concentration: 0.2-0.6 µg/mL. Additional bands of unknown identity were also consistently observed at 37 kDa and 26 kDa. These bands were successfully blocked by incubation with the immunising peptide.
IHC: In paraffin embedded Human Prostate shows nuclear staining of secretory cells. Recommended concentration: 5-10 µg/mL. Data obtained from a previous batch (different goat). This antibody has been successfully used for mouse embryonic stem cells.
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-FOXA1 antibody (Forkhead Box A1) (C-Term) (ABIN185021) ABIN185021 (0.2µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary...
Background publications Lin, Miller, Contreras et al.: "The hepatocyte nuclear factor 3 alpha gene, HNF3alpha (FOXA1), on chromosome band 14q13 is amplified and overexpressed in esophageal and lung adenocarcinomas." in: Cancer research, Vol. 62, Issue 18, pp. 5273-9, 2002 (PubMed).