Androgen Receptor (AR) (N-Term) antibody

Details for Product No. ABIN185266
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Antigen
Synonyms AR, NR3C4, AIS, DHTR, HUMARA, HYSP1, KD, SBMA, SMAX1, TFM, AW320017, Tfm, Andr
Epitope
N-Term
(37), (36), (32), (32), (23), (21), (17), (17), (17), (12), (8), (7), (7), (6), (6), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(411), (126), (120), (52), (51), (29), (24), (2), (2), (1), (1), (1)
Host
Goat
(349), (76), (7), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(18), (11), (11), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (3)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(270), (158), (116), (92), (80), (47), (34), (25), (24), (22), (9), (8), (6), (4), (4), (4), (3), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN185266
374.27 $
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Immunogen Peptide with sequence EVQLGLGRVYPRPPSC, from the N Terminus of the protein sequence according to NP_000035.2.
Specificity This antibody is expected to recognize isoform 1 (NP_000035.2) only.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Androgen Receptor
Background Synonyms: androgen receptor, dihydrotestosterone receptor, AR, KD, AIS, TFM, DHTR, SBMA, NR3C4, SMAX1, HUMARA, HYSP1, androgen receptor, dihydrotestosterone receptor, RP11-383C12.1
Gene ID 367, 11835, 24208
NCBI Accession NP_000035
Research Area Reproduction, Hormones, Receptors
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 95 kDa band observed in Human Brain and Human Heart lysates (calculated MW of 99 kDa according to NP_000035). Recommended concentration: 0.3-2 µg/mL.
IHC: In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Androgen Receptor (AR) (N-Term) antibody ABIN185266(0.3µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Trapman, Klaassen, Kuiper et al.: "Cloning, structure and expression of a cDNA encoding the human androgen receptor." in: Biochemical and biophysical research communications, Vol. 153, Issue 1, pp. 241-8, 1988 (PubMed).

Validation Images
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