AKT3 antibody

Antigen: AKT3

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185314

Additional Information

Gene ID: 10000

Immunogen: CSPTSQIDNIGEEEM

Format: Affinity-purified

Description: All names and symbols: AKT3, PKBG, PRKBG, STK-2, RAC-gamma, RAC-PK-gamma, DKFZP434N0250, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma), protein kinase B gamma, serine threonine protein kinase, Akt-3, RAC-gamma serine/threonine protein kinase. Official Symbol: AKT3. RefSeq number(s): NP_005456, NP_859029. Gene ontology terms: ATP binding, protein amino acid phosphorylation, protein serine/threonine kinase activity, signal transduction, transferase activity

Specificity: This antibody is expected to recognise an epitope corresponding to aa 119-133 of both isoforms of human AKT3 protein. This antibody does not cross-react with human AKT1/2. Human, Mouse, Rat 479 aa, 465 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: A 50-55kDa band observed in Human Hepatoblastoma HepG2 lysates (calculated MW of 55.8kDa according to NP_005456 and 54.0kDa according to NP_859029). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer

Restrictions: For Research Use only

Images

ABIN185314 (1µg/ml) staining of HepG2lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing AKT3 (RC224750) and probed with ABIN185314 (mock transfection in second lane), tested by Origene.

Publications

Publications: Zinda, Johnson, Paul et al.: "AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 7, Issue 8, pp. 2475-9, 2001 (PubMed).