Add to Basket
Order hotline:
 |
+1 404 474 4654 |
 |
+1 888 205 9894 (TF) |
AKT3 antibody
| Antigen | AKT3 |
| Clonality | Polyclonal |
| Host |
 Alternatives Goat |
| Reactivity |
Alternatives Human |
| Application |
Alternatives ELISA, Western Blotting (WB)
|
 |
1 reference available |
| Catalog no. | ABIN185314 |
| Quantity | 100 ug |
| Price | 501.80 $ Plus shipping costs $35.00 |
| Shipping to |
|
| Availability | Ships within 5 Business Days |
Additional Information
| Gene ID | 10000 |
| Immunogen | CSPTSQIDNIGEEEM |
| Format | Affinity-purified |
| Description | All names and symbols: AKT3, PKBG, PRKBG, STK-2, RAC-gamma, RAC-PK-gamma, DKFZP434N0250, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma), protein kinase B gamma, serine threonine protein kinase, Akt-3, RAC-gamma serine/threonine protein kinase. Official Symbol: AKT3. RefSeq number(s): NP_005456, NP_859029. Gene ontology terms: ATP binding, protein amino acid phosphorylation, protein serine/threonine kinase activity, signal transduction, transferase activity |
| Specificity | This antibody is expected to recognise an epitope corresponding to aa 119-133 of both isoforms of human AKT3 protein. This antibody does not cross-react with human AKT1/2. Human, Mouse, Rat 479 aa, 465 aa |
Application Details
| Application Notes | Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: A 50-55kDa band observed in Human Hepatoblastoma HepG2 lysates (calculated MW of 55.8kDa according to NP_005456 and 54.0kDa according to NP_859029). Recommended concentration: 1-3µg/ml. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Research Area | Cancer |
| Restrictions | For Research Use only |
Images
Publications
| Publications |
Zinda, Johnson, Paul et al.: "AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 7, Issue 8, pp. 2475-9, 2001 (PubMed).
|
Alternatives
Alternatives for antigen "AKT3", type "Antibodies"
| Hosts | Rabbit (32), Mouse (10), Goat (2), Chicken (1) |
| Reactivities | Human (41), Mouse (Murine) (16), Rat (Rattus) (16) |
| Applications | Western Blotting (WB) (26), ELISA (21), Immunofluorescence (IF) (21), Flow Cytometry (FACS) (19), Immunohistochemistry (IHC) (5), Immunoprecipitation (IP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoelectron Microscopy (IEM) (1) |
| Conjugates | Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1) |
| Epitopes | N-Term (1), S472 (1), T447 (1), internal (1) |
