AKT3 antibody (AA 119-133)

Catalog No. ABIN185314

This Goat Polyclonal antibody specifically detects AKT3 in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for AKT3 antibody (AA 119-133) (ABIN185314)

Target: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This AKT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-AKT3 Antibody

Binding Specificity: AA 119-133

Purpose: AKT3

Sequence: CSPTSQIDNI GEEEM

Specificity: This antibody is expected to recognise an epitope corresponding to aa 119-133 of both isoforms of human AKT3 protein. This antibody does not cross-react with human AKT1/2. Reported variants represent identical protein (NP_859029.1, NP_001193658.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CSPTSQIDNIGEEEM, from the internal region of the protein sequence according to NP_005456.1, NP_859029.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Prostate and Heart. Recommended concentration: 5 μg/mL.

Western Blot: A 50-55 kDa band observed in lysates of cell lines HepG2 and Jurkat and in Mouse and Rat Brain lysates (calculated MW of 54.0 kDa according to NP_859029.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AKT3

Target: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Alternative Name: AKT3

Background: AKT3, PKBG, PRKBG, STK-2, RAC-gamma, RAC-PK-gamma, DKFZP434N0250, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma), protein kinase B gamma, serine threonine protein kinase, Akt-3, RAC-gamma serine/threonine protein kinase, PKB-GAMMA

Gene ID: 10000

NCBI Accession: NP_005456, NP_859029

Pathways: PI3K-Akt Signaling, RTK Signaling, TLR Signaling, Hepatitis C, VEGF Signaling