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ERK2 antibody (Mitogen-Activated Protein Kinase 1) (Internal Region)

Details for Product anti-MAPK1 Antibody No. ABIN185327, Supplier: Log in to see
Antigen
  • 9030612K14Rik
  • AA407128
  • ATMPK1
  • ATMPK2
  • AU018647
  • C78273
  • erk
  • ERK
  • ERK2
  • Erk2
  • erk2
  • ert1
  • ERT1
  • F14N23.9
  • F14N23_9
  • mapk
  • mapk1
  • MAPK1
  • mapk1a
  • mapk1b
  • MAPK2
  • mapk2
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2
  • mitogen-activated protein kinase homolog 2
  • mpk1
  • p38
  • p40
  • p41
  • p41mapk
  • p42mapk
  • P42MAPK
  • Prkm1
  • prkm1
  • PRKM1
  • PRKM2
  • prkm2
  • T30E16.13
  • T30E16_13
  • Xp42
  • xp42
  • zERK2
Alternatives
anti-Human ERK2 antibody for Western Blotting
Epitope
Internal Region
62
24
15
9
8
8
7
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
317
101
69
34
17
7
5
3
3
3
2
2
2
1
1
1
1
Host
Goat
162
159
15
3
2
Clonality
Polyclonal
Conjugate
This ERK2 antibody is un-conjugated
8
8
7
7
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
301
140
85
81
67
52
51
26
17
10
5
3
2
2
2
1
1
1
1
Supplier
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Immunogen Peptide with sequence CAAGPEMVRGQVF, from the internal region of the protein sequence according to NP_002736.3, NP_620407.1.
Blocking Peptide Blocking peptide for this product available: ABIN368783
Specificity This antibody is expected to recognise an epitope corresponding to aa 9-19 of both reported protein isoforms of human ERK2 / MAPK1 protein.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ERK2 / MAPK1 (MAPK1 Antibody Abstract)
Background Synonyms: MAPK1, ERK2, ERK, p38, p40, p41, ERT1, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, mitogen-activated protein kinase 1, protein tyrosine kinase ERK2, mitogen-activated protein kinase 2, extracellular signal-regulated kinase 2
Gene ID 5594, 26413
NCBI Accession NP_002736, NP_620407
Pathways MAPK Signaling, RTK Signaling, Apoptosis, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 38 kDa band observed in human cervix epitheloid carcinoma HeLa and and human hepatoblastoma HepG2 lysates (calculated MW of 41.4 kDa according to NP_002736 and NP_620407). Recommended concentration: 0.5-2 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-ERK2 antibody (Mitogen-Activated Protein Kinase 1) (Internal Region) (ABIN185327) ABIN185327 (0.5µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary...
Background publications Lee, Nguyen, Park, Kim, Cho, Moon, Kim, Yoon, Hong: "EPO receptor-mediated ERK kinase and NF-kappaB activation in erythropoietin-promoted differentiation of astrocytes." in: Biochemical and biophysical research communications, Vol. 320, Issue 4, pp. 1087-95, 2004 (PubMed).

Favata, Horiuchi, Manos, Daulerio, Stradley, Feeser, Van Dyk, Pitts, Earl, Hobbs, Copeland, Magolda, Scherle, Trzaskos: "Identification of a novel inhibitor of mitogen-activated protein kinase kinase." in: The Journal of biological chemistry, Vol. 273, Issue 29, pp. 18623-32, 1998 (PubMed).