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CDKN1B antibody (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1)) (Internal Region)

Details for Product anti-CDKN1B Antibody No. ABIN185582, Supplier: Log in to see
Antigen
  • 1500011J20Rik
  • 2610202L11Rik
  • AA408329
  • AI843786
  • Bridge-1
  • Cdki1b
  • CDKN1B
  • cdkn1b
  • cdkn1bl
  • CDKN4
  • CIB
  • CIBP
  • FAM14D
  • ISG12
  • ISG12A
  • KIP1
  • Kip1
  • MEN1B
  • MEN4
  • P27
  • p27
  • p27Kip1
  • P27KIP1
  • PRKDCIP
  • sb:cb611
  • SIP2-28
  • wu:fb63g10
  • wu:fb64g10
  • wu:fe18e03
Alternatives
anti-Human CDKN1B antibody for Immunohistochemistry (Frozen Sections)
Epitope
Internal Region
111
56
51
44
34
27
22
21
21
20
20
20
19
17
15
14
14
13
13
9
8
8
8
6
6
6
6
5
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
709
330
274
93
46
22
17
9
8
6
6
3
2
2
2
2
1
1
Host
Goat
500
221
35
2
2
1
Clonality
Polyclonal
Conjugate
This CDKN1B antibody is un-conjugated
34
34
32
30
25
25
8
8
6
6
5
5
5
5
5
5
5
5
5
5
3
3
2
2
2
2
2
2
2
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
545
344
207
181
144
112
105
103
57
45
26
16
3
1
1
1
Supplier
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Immunogen Peptide with sequence C-KRPATDDSSTQNK, from the internal region of the protein sequence according to NP_004055.1.
Blocking Peptide Blocking peptide for this product available: ABIN369360
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cat (Feline), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name KIP1 / CDKN1B (CDKN1B Antibody Abstract)
Background Synonyms: CDKN1B, KIP1, cyclin-dependent kinase inhibitor 1B (p27, Kip1), CDKN4, P27KIP1, cyclin-dependent kinase inhibitor 1B
Gene ID 1027
NCBI Accession NP_004055
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 26 kDa band observed in lysates of Human Prostate and of Hepatoblastoma cell line HepG2 (calculated MW of 22.1 kDa according to NP_004055.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xia et al, Proc Natl Acad Sci U S A. 2005 Sep 27,102(39):14028-33, PMID: 16169901). Recommended concentration: 0.1-0.3 µg/mL.
IHC: In paraffin embedded Human Prostate shows nuclear staining of secretory cells. Recommended concentration: 5-10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-CDKN1B antibody (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1)) (Internal Region) (ABIN185582) ABIN185582 (0.1µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer)...
Background publications Baldassarre, Belletti, Nicoloso, Schiappacassi, Vecchione, Spessotto, Morrione, Canzonieri, Colombatti: "p27(Kip1)-stathmin interaction influences sarcoma cell migration and invasion." in: Cancer cell, Vol. 7, Issue 1, pp. 51-63, 2005 (PubMed).