STAT3 antibody (Internal Region)

Catalog No. ABIN185692

This Goat Polyclonal antibody specifically detects STAT3 in WB, IHC, ELISA and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for STAT3 antibody (Internal Region) (ABIN185692)

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This STAT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-STAT3 Antibody

Binding Specificity: Internal Region

Purpose: STAT3

Sequence: CRPESQEHPE ADP

Specificity: This antibody is expected to recognise all three reported isoforms (NP_644805.1, NP_003141.2 and NP_998827.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CRPESQEHPEADP, from the internal region of the protein sequence according to NP_644805.1, NP_003141.2, NP_998827.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 85 kDa band observed in lysates of cell line LNCaP, and approx. 90 kDa in Mouse Heart lysates (calculated MW of 83.1 kDa according to Human NP_998827.1 and 88.1 kDa according to Mouse : NP_998824.1). Recommended concentration: 0.5-3 μg/mL

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for STAT3

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Alternative Name: STAT3

Background: STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, FLJ20882, MGC16063 , DNA-binding protein APRF, acute-phase response factor, signal transducer and activator of transcription 3

Gene ID: 6774, 20848, 25125

NCBI Accession: NP_644805, NP_003141, NP_998827

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor