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Amphiregulin antibody (AA 20-100)

AREG Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal A6 unconjugated
Catalog No. ABIN1858071
  • Target See all Amphiregulin (AREG) Antibodies
    Amphiregulin (AREG)
    Binding Specificity
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 20-100
    Reactivity
    • 33
    • 12
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 28
    • 9
    • 1
    • 1
    • 1
    Mouse
    Clonality
    • 32
    • 8
    Monoclonal
    Conjugate
    • 28
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Amphiregulin antibody is un-conjugated
    Application
    • 27
    • 14
    • 10
    • 7
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Purpose
    Monoclonal Antibody to Amphiregulin (AREG)
    Sequence
    MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -S GHYAAGLDLN DTYSGKREPF SGDHSADGFE VTSRSEMSSG SEISPVSEMP SSSEPSSGAD YDYSEEYDNE PQIPGYIVDD
    Specificity
    The antibody is a mouse monoclonal antibody raised against AREG. It has been selected for its ability to recognize AREG in immunohistochemical staining and western blotting.
    Purification
    Protein A + Protein G affinity chromatography
    Immunogen
    Recombinant Amphiregulin (AREG) corresdonding to Ser20~Asp100 with N-terminal His and GST Tag
    Clone
    A6
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product AREG Primary Antibody
  • Application Notes

    Western blotting: 0.5-2 μg/mL

    Immunohistochemistry: 5-20 μg/mL

    Immunocytochemistry: 5-20 μg/mL

    Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Preservative
    ProClin
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freeze/thaw cycles
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    Amphiregulin (AREG)
    Alternative Name
    Amphiregulin (AREG Products)
    Synonyms
    AR antibody, CRDGF antibody, SDGF antibody, Sdgf antibody, AREG antibody, AREGB antibody, AREG/SDGF antibody, amphiregulin antibody, AREG antibody, Areg antibody
    Background
    AR, CRDGF, SDGF, Colorectum Cell-Derived Growth Factor, Schwannoma-Derived Growth Factor
    Pathways
    RTK Signaling, EGFR Signaling Pathway
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